PMID- 11407079
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20170112
IS  - 0392-856X (Print)
IS  - 0392-856X (Linking)
VI  - 19
IP  - 3
DP  - 2001 May-Jun
TI  - Evidence for hyaluronan production in the air pouch model in rats.
PG  - 271-6
AB  - OBJECTIVE: The aim of our work was to investigate the presence of hyaluronan (HA) 
      in the rat air pouch and its behaviour in response to inflammatory stimuli. 
      METHODS: HA levels (by a microplate assay) and the leucocyte count were 
      determined in the fluid obtained from air pouches in which acute or subacute 
      inflammation had been induced by the injection of monosodium urate crystals (MSU) 
      or high density polyethylene (HDPE) debris respectively and in relative controls. 
      RESULTS: In control pouches of both groups, remarkable levels of HA were found; 
      these levels were higher in the very first hours (2475 and 1850 micrograms/l at 6 
      hrs) and then gradually decreased. In pouches injected with MSU, HA moderately 
      increased (p < 0.001) after 6 hrs, reached a peak after 12 hrs (p < 0.001) and 
      began to taper at 24 hrs (p < 0.001). The leucocyte count was also increased at 6 
      hrs (p < 0.001), became higher at 12 hrs (p < 0.001) and tapered at 24 hrs (p < 
      0.001). In the HDPE pouches, HA levels were significantly reduced with respect to 
      controls after 6 hours (p < 0.001), increasing later (p < 0.001) to reach a peak 
      at 24 hrs (p < 0.001), and returning to the original levels, or even below, in 
      the following 72 hours. CONCLUSIONS: These data confirm that the pouch lining 
      produces fair amounts of HA and provide evidence that, in this system, HA levels 
      seem to be influenced by the degree of inflammation even if with variable 
      behaviour in relation to the different characteristics and phases of phlogosis. 
      The present data suggest that the air pouch is a useful experimental model for 
      studies on HA metabolism in either acute or chronic inflammation.
FAU - Generini, S
AU  - Generini S
AD  - Department of Internal Medicine, Section of Rheumatology, University of Florence, 
      Italy. generini@unifi.it
FAU - Matucci-Cerinic, M
AU  - Matucci-Cerinic M
FAU - Partsch, G
AU  - Partsch G
FAU - Stancikova, M
AU  - Stancikova M
FAU - Pignone, A
AU  - Pignone A
FAU - Konttinen, Y T
AU  - Konttinen YT
FAU - Rovensky, J
AU  - Rovensky J
FAU - Baker, D J
AU  - Baker DJ
FAU - Schumacher, H R Jr
AU  - Schumacher HR Jr
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Clin Exp Rheumatol
JT  - Clinical and experimental rheumatology
JID - 8308521
RN  - 268B43MJ25 (Uric Acid)
RN  - 9002-88-4 (Polyethylene)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Air
MH  - Animals
MH  - Body Fluids/immunology/metabolism
MH  - Disease Models, Animal
MH  - Hyaluronic Acid/*biosynthesis/*immunology
MH  - Inflammation/chemically induced/immunology/*metabolism
MH  - Leukocyte Count
MH  - Male
MH  - Polyethylene
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Uric Acid
EDAT- 2001/06/16 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/06/16 10:00
PHST- 2001/06/16 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/06/16 10:00 [entrez]
PST - ppublish
SO  - Clin Exp Rheumatol. 2001 May-Jun;19(3):271-6.