PMID- 11411506
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190605
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 63
IP  - 5
DP  - 2001 May
TI  - Polymerase chain reaction-based restriction fragment length polymorphism pattern 
      of porcine reproductive and respiratory syndrome virus directly from lung tissues 
      without virus isolation in Korea.
PG  - 567-71
AB  - Polymerase chain reaction (PCR)-based restriction fragment length polymorphism 
      (RFLP) analysis was developed for directly typing porcine reproductive and 
      respiratory syndrome virus (PRRSV) from lung specimens without virus isolation. 
      Twenty nine lung specimens collected from postweaning pigs were isolated for 
      PRRSV. When the PCR products from the 29 lung specimens were digested by the 
      restriction enzymes MluI, HincII, SacII and HaeIII, the RFLP patterns from the 29 
      lung specimens matched with those from the corresponding PRRSV isolates from each 
      pig. The results suggest that the PCR-based RFLP analysis method may be useful to 
      distinguish PRRSV isolates directly from lung specimens without virus isolation.
FAU - Cheon, D S
AU  - Cheon DS
AD  - Department of Veterinary Pathology, College of Veterinary Medicine and School of 
      Agricultural Biotechnology, Seoul National University, Kyounggi-Do, Republic of 
      Korea.
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA, Complementary/chemistry/genetics
MH  - Korea
MH  - Lung/*virology
MH  - Polymorphism, Restriction Fragment Length
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/chemistry/*genetics
MH  - RNA, Viral/chemistry/genetics/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/*veterinary
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2001/06/20 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/06/20 10:00
PHST- 2001/06/20 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/06/20 10:00 [entrez]
AID - 10.1292/jvms.63.567 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2001 May;63(5):567-71. doi: 10.1292/jvms.63.567.