PMID- 11411867
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20191210
IS  - 1121-760X (Print)
IS  - 1121-760X (Linking)
VI  - 45
IP  - 1
DP  - 2001
TI  - DNA aberrations in urinary bladder cancer detected by flow cytometry and FISH: 
      prognostic implications.
PG  - 65-71
AB  - We evaluated the genetic changes in bladder cancer biopsy by fluorescence in situ 
      hybridization (FISH) and related them to stage and grade of the tumor, ploidy 
      (FCM) and clinical outcome, to determine a simple method to identify tumors with 
      a poorer prognosis. Using FISH the numerical aberrations of chromosomes 1, 7, 9, 
      17 in tumor's imprints of 70 patients with transitional cell cancer (TCC) were 
      determined. First of all, the data demonstrated that the sensitivity of FISH in 
      detecting quantitative DNA aberrations exceeds FCM's sensitivity. The frequency 
      of chromosome 1 and 9 aberrations did not show significant differences in diploid 
      and aneuploid tumors in different stage and grade. On the contrary, the 
      chromosome 7 and 17 aneusomy showed greater differences between pT1 and pT2-3 
      tumors (p<0.032 and p<0.0006, respectively) than between stage pTa and pT1. In 
      our investigation, an increasing number of aberrations was observed in all 
      chromosomes examined in tumors of patients who afterwards underwent cystectomy 
      and/or had recurrent tumors. These results suggest that chromosome 7 and 17 
      aneusomy could be predictive of adverse outcome in a subgroup of patients with 
      superficial tumors at presentation.
FAU - Cianciulli, A M
AU  - Cianciulli AM
AD  - Clinical Pathology Service, National Cancer Institute, Rome. cianciulli@ifo.it
FAU - Bovani, R
AU  - Bovani R
FAU - Leonardo, C
AU  - Leonardo C
FAU - Iori, F
AU  - Iori F
FAU - Coletta, A M
AU  - Coletta AM
FAU - Marzano, R
AU  - Marzano R
FAU - Antenucci, A
AU  - Antenucci A
FAU - Gandolfo, G M
AU  - Gandolfo GM
FAU - Laurenti, C
AU  - Laurenti C
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - Italy
TA  - Eur J Histochem
JT  - European journal of histochemistry : EJH
JID - 9207930
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/*genetics/pathology/surgery
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local
MH  - Ploidies
MH  - Prognosis
MH  - Sensitivity and Specificity
MH  - Urinary Bladder Neoplasms/*genetics/pathology/surgery
EDAT- 2001/06/20 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/06/20 10:00
PHST- 2001/06/20 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/06/20 10:00 [entrez]
AID - 10.4081/1615 [doi]
PST - ppublish
SO  - Eur J Histochem. 2001;45(1):65-71. doi: 10.4081/1615.