PMID- 11416843
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20190910
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 57
IP  - 1
DP  - 2001 Oct
TI  - Poly(DL-lactic-co-glycolic acid) sponge hybridized with collagen microsponges and 
      deposited apatite particulates.
PG  - 8-14
AB  - A novel three-dimensional porous scaffold has been developed for bone tissue 
      engineering by hybridizing synthetic poly(DL-lactic-co-glycolic acid) (PLGA), 
      naturally derived collagen, and inorganic apatite. First, a porous PLGA sponge 
      was prepared. Then, collagen microsponges were formed in the pores of the PLGA 
      sponge. Finally, apatite particulates were deposited on the surfaces of the 
      collagen microsponges in the pores of PLGA sponge. The PLGA-collagen sponge 
      served as a template for apatite deposition, and the deposition was accomplished 
      by alternate immersion of PLGA-collagen sponge in CaCl(2) and Na(2)HPO(4) aqueous 
      solutions and centrifugation. The deposited particulates were small and scarce 
      after one cycle of alternate immersion. Their number and size increased with the 
      number of alternate immersion cycles. The surfaces of collagen microsponges were 
      completely covered with apatite after three cycles of alternate immersion. The 
      porosity of the hybrid sponge decreased gradually as the number of alternate 
      immersion increased. Energy-dispersive spectroscopy analysis and X-ray 
      diffraction spectra showed that the calcium-to-phosphorus molar ratio of the 
      deposited particulates and the level of crystallinity increased with the number 
      of alternate immersion cycles, and became almost the same as that of 
      hydroxyapatite after four cycles of alternate immersion. The deposition process 
      was controllable. Use of the PLGA sponge as a mechanical skeleton facilitated 
      formation of the PLGA-collagen-apatite hybrid sponge into desired shapes and 
      collagen microsponges facilitated the uniform deposition of apatite particulates 
      throughout the sponge. The PLGA-collagen-apatite hybrid sponge would serve as a 
      useful three-dimensional porous scaffold for bone tissue engineering.
FAU - Chen, G
AU  - Chen G
AD  - Tissue Engineering Research Center, National Institute of Advanced Industrial 
      Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, 
      Japan. guoping-chen@aist.go.jp
FAU - Ushida, T
AU  - Ushida T
FAU - Tateishi, T
AU  - Tateishi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Apatites)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Apatites/chemistry
MH  - Biocompatible Materials/*chemistry
MH  - Collagen/*chemistry
MH  - Lactic Acid/*chemistry
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry
EDAT- 2001/06/21 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/06/21 10:00
PHST- 2001/06/21 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/06/21 10:00 [entrez]
AID - 10.1002/1097-4636(200110)57:1<8::AID-JBM1135>3.0.CO;2-H [pii]
AID - 10.1002/1097-4636(200110)57:1<8::aid-jbm1135>3.0.co;2-h [doi]
PST - ppublish
SO  - J Biomed Mater Res. 2001 Oct;57(1):8-14. doi: 
      10.1002/1097-4636(200110)57:1<8::aid-jbm1135>3.0.co;2-h.