PMID- 11418611
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 32
DP  - 2001 Aug 10
TI  - RGS2 promotes adipocyte differentiation in the presence of ligand for peroxisome 
      proliferator-activated receptor gamma.
PG  - 29625-7
AB  - The events at the earliest stage of adipocyte differentiation are yet to be fully 
      elucidated. Previously, we cloned the genes that are induced at the beginning of 
      the differentiation of mouse 3T3-L1 preadipocyte cells. We found that the gene 
      expression of regulators of G protein signaling-2 (RGS2) rapidly increased after 
      the addition of inducers and decreased at 3-12 h. The expression pattern of RGS2 
      mRNAs differed among growth-arrested and proliferating 3T3-L1 cells and NIH-3T3 
      cells, indicating a specificity for adipogenesis. Here we report that the ectopic 
      expression of RGS2 using a retroviral system in mouse NIH-3T3 cells promotes 
      adipogenesis only in the presence of BRL49653, which is a ligand for the 
      peroxisome proliferator-activated receptor gamma (PPARgamma). These results 
      strongly suggest that RGS2 play a crucial role in the program of adipocyte 
      differentiation and may contribute to the function of PPARgamma.
FAU - Nishizuka, M
AU  - Nishizuka M
AD  - Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical 
      Sciences, Osaka University, 1-6 Yamada-Oka, Suita, Osaka 565-0871, Japan.
FAU - Honda, K
AU  - Honda K
FAU - Tsuchiya, T
AU  - Tsuchiya T
FAU - Nishihara, T
AU  - Nishihara T
FAU - Imagawa, M
AU  - Imagawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010620
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Ligands)
RN  - 0 (RGS Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Rgs2 protein, mouse)
RN  - 0 (Thiazoles)
RN  - 0 (Thiazolidinediones)
RN  - 0 (Transcription Factors)
RN  - 0 (Vasodilator Agents)
RN  - 05V02F2KDG (Rosiglitazone)
SB  - IM
MH  - 3T3 Cells
MH  - Adipocytes/*cytology/metabolism
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Differentiation
MH  - *Ligands
MH  - Mice
MH  - Plasmids/metabolism
MH  - RGS Proteins/*physiology
MH  - RNA, Messenger/metabolism
MH  - Receptors, Cytoplasmic and Nuclear/*metabolism
MH  - Rosiglitazone
MH  - Thiazoles/pharmacology
MH  - *Thiazolidinediones
MH  - Time Factors
MH  - Transcription Factors/*metabolism
MH  - Vasodilator Agents/pharmacology
EDAT- 2001/06/22 10:00
MHDA- 2001/09/08 10:01
CRDT- 2001/06/22 10:00
PHST- 2001/06/22 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/06/22 10:00 [entrez]
AID - S0021-9258(20)89653-7 [pii]
AID - 10.1074/jbc.C100272200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 Aug 10;276(32):29625-7. doi: 10.1074/jbc.C100272200. Epub 2001 
      Jun 20.