PMID- 11419704
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20190901
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 756
IP  - 1-2
DP  - 2001 May 25
TI  - Immunological identification and characterization of individual food allergens.
PG  - 131-40
AB  - Food allergies of type-I-allergy are immunoglobulin E (IgE) mediated and caused 
      by certain proteins or glycoproteins, which are called food allergens. An 
      analytical marker of allergens is the IgE-reactivity to these substances. 
      Normally food allergens are minor components in allergenic source material, which 
      consist of a huge number of chemical different substances. Thus allergen 
      extraction, separation and immunological detection methods are described which 
      identify and characterize individual food allergens by a minimum of manipulation. 
      Favoured separation methods of allergenic extracts are electrophoretic ones 
      allowing the combination of highly resolved protein separations with 
      immunological detection methods subsumed by the term immunoblotting. These 
      techniques are a useful basis to characterize allergens by chemical methods. Once 
      the primary protein structure of a food allergen is established, the way is 
      cleared for the identification of epitopes. Epitopes are immunological detectable 
      parts of a protein or glycoprotein generating the interface between chemical 
      structure and immune-system. The nature of epitopes may differ, for instance, can 
      be conformational, continuous, or built up by glycoconjugates, which determine 
      the stability of food allergens, especially in the case of food processing. 
      Progress in identification and characterization of food allergens will improve 
      diagnostics and therapy of food allergy.
FAU - Becker, W M
AU  - Becker WM
AD  - Forschungszentrum Borstel, Biochemische und Molekulare Allergologie, Germany. 
      wbecker@fz-borstel.de
FAU - Reese, G
AU  - Reese G
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Allergens)
RN  - 0 (Epitopes)
SB  - IM
MH  - Allergens/*chemistry/immunology/isolation & purification
MH  - Blotting, Western
MH  - Epitopes/chemistry
MH  - *Food Hypersensitivity
RF  - 30
EDAT- 2001/06/23 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/06/23 10:00
PHST- 2001/06/23 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/06/23 10:00 [entrez]
AID - 10.1016/s0378-4347(01)00098-6 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 2001 May 25;756(1-2):131-40. doi: 
      10.1016/s0378-4347(01)00098-6.