PMID- 11420155
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20191025
IS  - 0967-2109 (Print)
IS  - 0967-2109 (Linking)
VI  - 9
IP  - 4
DP  - 2001 Aug
TI  - Expression of apoptosis-related proteins and structural features of cell death in 
      explanted aortocoronary saphenous vein bypass grafts.
PG  - 319-28
AB  - This study aimed to investigate the features of cell death occurring in 
      aortocoronary saphenous vein bypass grafts. Human aortocoronary saphenous vein 
      bypass grafts with angiographic luminal stenosis of > 75% were explanted from 14 
      patients at redo coronary artery bypass grafting. Proteins associated with 
      apoptotic pathways were identified immunohistochemically using antibodies to 
      Bcl-2, Fas, BAX, p53 and CPP32. Cells undergoing DNA fragmentation were 
      identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL). DNA synthesis was investigated using the antibody to 
      proliferating cell nuclear antigen (PCNA). Ultrastructural features of cell death 
      were examined by electron microscopy. Anti-apoptotic (Bcl-2) and pro-apoptotic 
      (Bax, p53, CPP32 and Fas) proteins were expressed throughout the graft wall, but 
      marked differences in the characteristics of cell death were noted between 
      atherosclerotic and non-atherosclerotic areas of the intima. In atherosclerotic 
      areas, pro-apoptotic proteins were widely expressed, but ultrastructural analysis 
      failed to identify cells showing typical features of apoptosis. In these areas, 
      necrotic cells were frequently observed, with negative correlation of Bcl-2 
      expression with TUNEL. Pro-apoptotic proteins showed no correlation with TUNEL. 
      In contrast, in non-atherosclerotic areas of vein grafts, the expression of both 
      anti-apoptotic (Bcl-2) and pro-apoptotic proteins (p53, Bax and CPP32) correlated 
      with TUNEL. In atherosclerotic areas, non-atherosclerotic intimal areas, and in 
      the underlying media, the numbers of TUNEL+ cells correlated with PCNA 
      positivity. Ultrastructurally, apoptotic bodies and features of necrosis were 
      observed in non-atherosclerotic areas of grafts. The present observations 
      indicate that in atherosclerotic areas, cell death occurs mainly by necrosis, 
      while in non-atherosclerotic areas, cell death occurs by both necrosis and 
      apoptosis. An imbalance between DNA fragmentation and DNA synthesis may 
      contribute to graft instability and failure.
FAU - Wang, A Y
AU  - Wang AY
AD  - Surgical Professorial Unit, St Vincent's Hospital, Victoria Street, Darlinghurst, 
      NSW 2010, Sydney, Australia.
FAU - Bobryshev, Y V
AU  - Bobryshev YV
FAU - Cherian, S M
AU  - Cherian SM
FAU - Liang, H
AU  - Liang H
FAU - Tran, D
AU  - Tran D
FAU - Inder, S J
AU  - Inder SJ
FAU - Lord, R S
AU  - Lord RS
FAU - Ashwell, K W
AU  - Ashwell KW
FAU - Farnsworth, A E
AU  - Farnsworth AE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cardiovasc Surg
JT  - Cardiovascular surgery (London, England)
JID - 9308765
RN  - 0 (BAX protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (fas Receptor)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Aged
MH  - Apoptosis/*genetics
MH  - Caspase 3
MH  - Caspases/genetics
MH  - Cell Death/*genetics
MH  - *Coronary Artery Bypass
MH  - Coronary Artery Disease/genetics/pathology/*surgery
MH  - Female
MH  - Gene Expression/physiology
MH  - Graft Occlusion, Vascular/*genetics/pathology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Necrosis
MH  - Proto-Oncogene Proteins/genetics
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - Reoperation
MH  - Tumor Suppressor Protein p53/genetics
MH  - Veins/pathology/*transplantation
MH  - bcl-2-Associated X Protein
MH  - fas Receptor/genetics
EDAT- 2001/06/23 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/06/23 10:00
PHST- 2001/06/23 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/06/23 10:00 [entrez]
AID - S0967210900001253 [pii]
AID - 10.1016/s0967-2109(00)00125-3 [doi]
PST - ppublish
SO  - Cardiovasc Surg. 2001 Aug;9(4):319-28. doi: 10.1016/s0967-2109(00)00125-3.