PMID- 11420179
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1532
IP  - 1-2
DP  - 2001 May 31
TI  - Structure, expression profile and alternative processing of the human 
      phosphatidylethanolamine N-methyltransferase (PEMT) gene.
PG  - 105-14
AB  - Phosphatidylethanolamine N-methyltransferase (PEMT) catalyzes the conversion of 
      phosphatidylethanolamine (PE) to phosphatidylcholine (PC) in a series of three 
      methylation reactions. Preliminary studies of PEMT in humans led to the cloning 
      of three cDNAs each of which has a different 5' untranslated region (5'UTR). To 
      determine the origin of PEMT splice variants and to investigate expression of the 
      gene in human liver, we isolated a bacterial artificial chromosome (BAC) clone 
      containing the full-length human gene. Each of the three unique untranslated 
      first exons is present in a contiguous array in the gene, confirming the 
      integrity of the cDNAs and alternative processing of PEMT transcripts. Human 
      liver, heart and testis contain the highest levels of PEMT transcripts and of 
      these, liver has the greatest PEMT expression. Furthermore, each of the three 
      PEMT transcripts is present in varying abundance in liver whereas heart and 
      testis contain only one and two transcripts, respectively. Thus, differential 
      promoter usage in the human PEMT gene generates three unique transcripts and 
      confers a tissue-specific expression pattern.
FAU - Shields, D J
AU  - Shields DJ
AD  - Department of Biochemistry and CIHR Group on Molecular and Cell Biology of 
      Lipids, University of Alberta, 328 Heritage Medical Research Building, T6G 2S2, 
      Edmonton, AB, Canada.
FAU - Agellon, L B
AU  - Agellon LB
FAU - Vance, D E
AU  - Vance DE
LA  - eng
SI  - GENBANK/AF294460
SI  - GENBANK/AF294461
SI  - GENBANK/AF294462
SI  - GENBANK/AF294463
SI  - GENBANK/AF294464
SI  - GENBANK/AF294465
SI  - GENBANK/AF294466
SI  - GENBANK/AF294467
SI  - GENBANK/AF294468
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.17 (PEMT protein, human)
RN  - EC 2.1.1.17 (Phosphatidylethanolamine N-Methyltransferase)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Base Sequence
MH  - Chromosomes, Artificial, Bacterial
MH  - Cloning, Molecular
MH  - DNA Methylation
MH  - DNA, Complementary
MH  - Exons
MH  - Humans
MH  - Methyltransferases/*genetics
MH  - Molecular Sequence Data
MH  - Phosphatidylethanolamine N-Methyltransferase
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
EDAT- 2001/06/23 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/06/23 10:00
PHST- 2001/06/23 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/06/23 10:00 [entrez]
AID - S1388-1981(01)00122-6 [pii]
AID - 10.1016/s1388-1981(01)00122-6 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2001 May 31;1532(1-2):105-14. doi: 
      10.1016/s1388-1981(01)00122-6.