PMID- 11420220
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 65
IP  - 1
DP  - 2001 Jul
TI  - Expression of matrix metalloproteinase-2, -9, -14, and tissue inhibitors of 
      metalloproteinase-1, -2, -3 in the endometrium and placenta of rhesus monkey 
      (Macaca mulatta) during early pregnancy.
PG  - 31-40
AB  - The extracellular matrix proteolytic machinery has long been recognized as one of 
      the most important mechanisms for regulating trophoblast invasion. Matrix 
      metalloproteinases (MMPs) are a group of proteases involved in this process, and 
      their activities are regulated by tissue inhibitors of MMPs (TIMPs). In this 
      study, we collected rhesus monkey uteri on Days 12, 18, and 26 of pregnancy and 
      examined the mRNA expression of MMP-2, -9, -14, and TIMP-1, -2, -3, as well as 
      the activities of MMP-2 and -9 by using in situ hybridization and gelatin 
      zymography, respectively. The results showed that MMP-2 and -9 were expressed 
      earlier than MMP-14 and TIMPs in the pregnant endometrium. MMP-14 and TIMP-2 
      mRNAs appeared in perivascular decidual cells earlier than MMP-2 mRNA. On Day 26 
      of pregnancy, placental villi expressed little MMP-2, -14, and TIMP transcripts 
      but abundant MMP-9 mRNA. Furthermore, MMP-2, -9, -14, and TIMP-1, -2, -3 were 
      highly expressed on the fetal-maternal border but were absent in the myometrium. 
      TIMP-3 mRNA in the endometrium was specifically localized to some cells lining 
      the outer membrane of several groups of arterioles. Combined with the results 
      obtained by gelatin zymography, we found that active MMP-2 existed in the 
      endometrium throughout these three phases, while MMP-9 showed considerable 
      activities only on Days 18 and 26 of pregnancy. The data suggest key roles for 
      MMP-2 and -9 in invasion of trophoblast cells into the endometrium and the 
      development of the placenta and might indicate that these processes are regulated 
      by MMP-14 and TIMP-1, -2, and -3.
FAU - Wang, H
AU  - Wang H
AD  - State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese 
      Academy of Sciences, Beijing 100080, China.
FAU - Li, Q
AU  - Li Q
FAU - Shao, L
AU  - Shao L
FAU - Zhu, C
AU  - Zhu C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Matrix Metalloproteinases, Membrane-Associated)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Endometrium/anatomy & histology/*metabolism
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Macaca mulatta
MH  - Matrix Metalloproteinase 2/*biosynthesis
MH  - Matrix Metalloproteinase 9/*biosynthesis
MH  - Matrix Metalloproteinases, Membrane-Associated
MH  - Metalloendopeptidases/*biosynthesis
MH  - Placenta/anatomy & histology/*metabolism
MH  - Pregnancy
MH  - RNA, Messenger/biosynthesis
MH  - Tissue Inhibitor of Metalloproteinase-1/*biosynthesis
MH  - Tissue Inhibitor of Metalloproteinase-2/*biosynthesis
MH  - Tissue Inhibitor of Metalloproteinase-3/*biosynthesis
MH  - Uterus/anatomy & histology/metabolism
EDAT- 2001/06/23 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/06/23 10:00
PHST- 2001/06/23 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/06/23 10:00 [entrez]
AID - 10.1095/biolreprod65.1.31 [doi]
PST - ppublish
SO  - Biol Reprod. 2001 Jul;65(1):31-40. doi: 10.1095/biolreprod65.1.31.