PMID- 11420462
OWN - NLM
STAT- MEDLINE
DCOM- 20010726
LR  - 20190822
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 25
IP  - 7
DP  - 2001 Jul
TI  - Analysis of Hurthle cell neoplasms of the thyroid by interphase fluorescence in 
      situ hybridization.
PG  - 911-7
AB  - Recent studies have indicated that numerical chromosomal abnormalities including 
      changes in p53 and cyclin D1 may be involved in Hurthle cell tumorigenesis. We 
      analyzed a series of Hurthle cell neoplasms of the thyroid to evaluate the 
      diagnostic and prognostic utility of numerical anomalies by DNA fluorescent 
      probes for cyclin D1 and p53 gene loci and chromosomes 5, 7, 11, 12, 17, and 22. 
      Interphase fluorescence in situ hybridization (FISH) analysis was performed on 
      paraffin-embedded tissue sections from 10 Hurthle cell adenomas, 19 Hurthle cell 
      carcinomas, and 7 normal thyroid tissues used as controls. Directly labeled 
      fluorescent DNA probes for the centromere region of chromosomes 7, 11, 12, and 17 
      and locus-specific probes for chromosomes 5 and 22, cyclin D1, and p53 were 
      utilized for dual-probe hybridizations. Sixty percent (6 of 10) Hurthle cell 
      adenomas and 63% (12 of 19) Hurthle cell carcinomas showed chromosome gains. 
      Twenty percent (2 of 10) Hurthle cell adenomas and 26% (5 of 19) Hurthle cell 
      carcinomas showed chromosome losses. Normal thyroid tissues used as controls 
      showed no chromosomal abnormalities. Among Hurthle cell tumors with chromosomal 
      abnormalities, adenomas averaged 2.7 gains and 0.3 losses per case, and 
      carcinomas averaged 3.3 gains and 0.6 losses per case. The two adenomas with 
      chromosome losses each showed loss of one chromosome, whereas the five carcinomas 
      with losses averaged 1.8 losses per case. Chromosome 22 was the most common loss 
      identified, occurring in three of the 11 patients who died of disease. These 
      results indicate that chromosomal imbalances as gains are common in both benign 
      and malignant Hurthle cell neoplasms, but Hurthle cell carcinomas tend to have 
      more chromosome losses than adenomas. Among Hurthle cell carcinomas in this 
      study, chromosome losses were identified only from patients who died of disease. 
      The loss of chromosome 22 may have prognostic value in Hurthle cell carcinoma of 
      the thyroid.
FAU - Erickson, L A
AU  - Erickson LA
AD  - Departments of Laboratory Medicine and Pathology, Mayo Clinic and Mayo 
      Foundation, Rochester, Minnesota, USA.
FAU - Jalal, S M
AU  - Jalal SM
FAU - Goellner, J R
AU  - Goellner JR
FAU - Law, M E
AU  - Law ME
FAU - Harwood, A
AU  - Harwood A
FAU - Jin, L
AU  - Jin L
FAU - Roche, P C
AU  - Roche PC
FAU - Lloyd, R V
AU  - Lloyd RV
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adenoma, Oxyphilic/diagnosis/genetics/*pathology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Chromosome Disorders
MH  - Chromosome Mapping
MH  - Cyclin D1/genetics
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Thyroid Neoplasms/diagnosis/genetics/*pathology
MH  - Tumor Suppressor Protein p53/genetics
EDAT- 2001/06/23 10:00
MHDA- 2001/07/28 10:01
CRDT- 2001/06/23 10:00
PHST- 2001/06/23 10:00 [pubmed]
PHST- 2001/07/28 10:01 [medline]
PHST- 2001/06/23 10:00 [entrez]
AID - 10.1097/00000478-200107000-00009 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2001 Jul;25(7):911-7. doi: 10.1097/00000478-200107000-00009.