PMID- 11420745
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20220321
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Electronic)
IS  - 1476-5586 (Linking)
VI  - 3
IP  - 2
DP  - 2001 Mar-Apr
TI  - Detection of MYCN gene amplification in neuroblastoma by fluorescence in situ 
      hybridization: a pediatric oncology group study.
PG  - 105-9
AB  - To assess the utility of fluorescence in situ hybridization (FISH) for analysis 
      of MYCN gene amplification in neuroblastoma, we compared this assay with Southern 
      blot analysis using tumor specimens collected from 232 patients with presenting 
      characteristics typical of this disease. The FISH technique identified MYCN 
      amplification in 47 cases, compared with 39 by Southern blotting, thus increasing 
      the total number of positive cases by 21%. The major cause of discordancy was a 
      low fraction of tumor cells (< or =30% replacement) in clinical specimens, which 
      prevented an accurate estimate of MYCN copy number by Southern blotting. With 
      FISH, by contrast, it was possible to analyze multiple interphase nuclei of tumor 
      cells, regardless of the proportion of normal peripheral blood, bone marrow, or 
      stromal cells in clinical samples. Thus, FISH could be performed accurately with 
      very small numbers of tumor cells from touch preparations of needle biopsies. 
      Moreover, this procedure allowed us to discern the heterogeneous pattern of MYCN 
      amplification that is characteristic of neuroblastoma. We conclude that FISH 
      improves the detection of MYCN gene amplification in childhood neuroblastomas in 
      a clinical setting, thus facilitating therapeutic decisions based on the presence 
      or absence of this prognostically important biologic marker.
FAU - Mathew, P
AU  - Mathew P
AD  - Department of Experimental Oncology, St. Jude Children's Research Hospital, 
      Memphis, TN, USA.
FAU - Valentine, M B
AU  - Valentine MB
FAU - Bowman, L C
AU  - Bowman LC
FAU - Rowe, S T
AU  - Rowe ST
FAU - Nash, M B
AU  - Nash MB
FAU - Valentine, V A
AU  - Valentine VA
FAU - Cohn, S L
AU  - Cohn SL
FAU - Castleberry, R P
AU  - Castleberry RP
FAU - Brodeur, G M
AU  - Brodeur GM
FAU - Look, A T
AU  - Look AT
LA  - eng
GR  - P30 CA021765/CA/NCI NIH HHS/United States
GR  - CA 21765/CA/NCI NIH HHS/United States
GR  - CA 31566/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
RN  - 0 (Proto-Oncogene Proteins c-myc)
SB  - IM
MH  - Blotting, Southern/*methods
MH  - Child, Preschool
MH  - Female
MH  - Genes, myc/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant
MH  - Male
MH  - Neuroblastoma/diagnosis/*genetics/*metabolism
MH  - Proto-Oncogene Proteins c-myc/*biosynthesis
PMC - PMC1505416
EDAT- 2001/06/23 10:00
MHDA- 2001/09/28 10:01
PMCR- 2001/03/01
CRDT- 2001/06/23 10:00
PHST- 2000/09/27 00:00 [received]
PHST- 2000/10/02 00:00 [accepted]
PHST- 2001/06/23 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/06/23 10:00 [entrez]
PHST- 2001/03/01 00:00 [pmc-release]
AID - 0146a [pii]
AID - 10.1038/sj.neo.7900146 [doi]
PST - ppublish
SO  - Neoplasia. 2001 Mar-Apr;3(2):105-9. doi: 10.1038/sj.neo.7900146.