PMID- 11422197
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20190513
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 124
IP  - 2
DP  - 2001 May
TI  - Mechanism of NK cell activation induced by coculture with dendritic cells derived 
      from peripheral blood monocytes.
PG  - 214-22
AB  - Dendritic cells (DCs) have been regarded as one of the effective 
      antigen-presenting cells, but the relationship between DCs and lymphocytes, in 
      particular natural killer (NK) cells, remains unclear. In this study, we 
      evaluated how DCs interact with both lymphocytes and NK cells using a coculture 
      system. The number of lymphocytes increased significantly when cocultured with 
      DCs (1.8-fold increase). In particular, the proliferation of NK cells was 
      prominent. Furthermore, the coculture of DCs with lymphocytes induced a marked 
      increase in IL-12 and IFN-gamma secretion. When contact between the DCs and 
      lymphocytes was prevented, the secretion of both IL-12 and IFN-gamma was markedly 
      reduced. IFN-gamma production was completely blocked by an anti-IL-12 antibody, 
      indicating that IFN-gamma secretion was dependent on IL-12 secretion. The 
      stimulating effect of the DCs on the proliferation of the lymphocytes was 
      partially suppressed by anti-IL-12 antibodies, and was completely attenuated when 
      cellular contact was prevented. Furthermore, the NK cell proliferation induced by 
      coculture with DCs was significantly blocked by the inhibition of the interaction 
      of either CD40-CD40L or CD28-B7 molecule. The coculture with DCs enhanced NK 
      activity by 40%, and this was partially suppressed by anti-IL-12 antibodies and 
      was completely blocked by the inhibition of cell-to-cell contact. These results 
      indicate that the activation of NK cells by DCs is partially mediated by IL-12 
      secretion, and that direct contact between DCs and NK cells play a major role in 
      this response.
FAU - Amakata, Y
AU  - Amakata Y
AD  - Department of Internal Medicine, Shiga University of Medical Science, 
      Seta-Tukinowa, Ostu, Japan. andoh@belle.shiga-med.ac.jp
FAU - Fujiyama, Y
AU  - Fujiyama Y
FAU - Andoh, A
AU  - Andoh A
FAU - Hodohara, K
AU  - Hodohara K
FAU - Bamba, T
AU  - Bamba T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (CD56 Antigen)
RN  - 0 (Receptors, IgG)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - CD56 Antigen/isolation & purification
MH  - Coculture Techniques
MH  - Dendritic Cells/cytology/*immunology
MH  - Humans
MH  - Interferon-gamma
MH  - Interleukin-12
MH  - Killer Cells, Natural/cytology/*immunology
MH  - Monocytes/cytology/*immunology
MH  - Receptors, IgG/isolation & purification
MH  - T-Lymphocytes, Cytotoxic/cytology/*immunology
PMC - PMC1906048
EDAT- 2001/06/26 10:00
MHDA- 2001/07/13 10:01
PMCR- 2002/05/01
CRDT- 2001/06/26 10:00
PHST- 2001/06/26 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/06/26 10:00 [entrez]
PHST- 2002/05/01 00:00 [pmc-release]
AID - cei1550 [pii]
AID - 10.1046/j.1365-2249.2001.01550.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2001 May;124(2):214-22. doi: 10.1046/j.1365-2249.2001.01550.x.