PMID- 11424007
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20071114
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 184
IP  - 2
DP  - 2001 Jul 15
TI  - Definition of an optimal cytotoxic T lymphocyte epitope in the latently expressed 
      Kaposi's sarcoma-associated herpesvirus kaposin protein.
PG  - 119-26
AB  - Cytotoxic T lymphocytes (CTL) recognize and kill virus-infected cells and 
      contribute to immunologic control of viral replication. For many herpesviruses 
      (e.g., Epstein-Barr and cytomegalovirus), virus-specific CTL responses can be 
      readily detected in infected persons, but CTL responses against Kaposi's 
      sarcoma-associated herpesvirus (KSHV) appear to be weak and remain poorly 
      characterized. Using a human leukocyte antigen (HLA) binding motif-based epitope 
      prediction algorithm, we identified 37 HLA-A*0201 binding peptides from 8 KSHV 
      open-reading frames (ORFs). After in vitro stimulation of peripheral blood 
      mononuclear cells from KSHV-infected persons, CTL responses against 1 peptide in 
      the KSHV kaposin protein (ORF K12) were detected in 2 HLA-A*0201-positive 
      subjects. The optimal CTL epitope was identified by HLA restriction analysis and 
      peptide titration assays. These data describe a latent phase viral gene product 
      targeted by CTL that may be relevant for KSHV immunopathogenesis.
FAU - Brander, C
AU  - Brander C
AD  - AIDS Research Center and Cancer Center, Massachusetts General Hospital and 
      Harvard Medical School, Boston, MA, USA.
FAU - O'Connor, P
AU  - O'Connor P
FAU - Suscovich, T
AU  - Suscovich T
FAU - Jones, N G
AU  - Jones NG
FAU - Lee, Y
AU  - Lee Y
FAU - Kedes, D
AU  - Kedes D
FAU - Ganem, D
AU  - Ganem D
FAU - Martin, J
AU  - Martin J
FAU - Osmond, D
AU  - Osmond D
FAU - Southwood, S
AU  - Southwood S
FAU - Sette, A
AU  - Sette A
FAU - Walker, B D
AU  - Walker BD
FAU - Scadden, D T
AU  - Scadden DT
LA  - eng
GR  - AI-42851/AI/NIAID NIH HHS/United States
GR  - CA-73580/CA/NCI NIH HHS/United States
GR  - CA-78124/CA/NCI NIH HHS/United States
GR  - CA-78378/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20010618
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (Viral Proteins)
RN  - 0 (kaposin B protein, Human herpesvirus 8)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*immunology
MH  - *Epitopes
MH  - Gene Expression Regulation, Viral
MH  - HLA-A Antigens/*immunology
MH  - Herpesvirus 8, Human/*immunology
MH  - Humans
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Viral Proteins/*immunology
EDAT- 2001/06/26 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/06/26 10:00
PHST- 2001/03/06 00:00 [received]
PHST- 2001/04/05 00:00 [revised]
PHST- 2001/06/26 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/06/26 10:00 [entrez]
AID - JID010129 [pii]
AID - 10.1086/322003 [doi]
PST - ppublish
SO  - J Infect Dis. 2001 Jul 15;184(2):119-26. doi: 10.1086/322003. Epub 2001 Jun 18.