PMID- 11424011
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20131121
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 184
IP  - 2
DP  - 2001 Jul 15
TI  - The importance of a beta-glucan receptor in the nonopsonic entry of nontypeable 
      Haemophilus influenzae into human monocytic and epithelial cells.
PG  - 150-8
AB  - Previous reports showed that nontypeable Haemophilus influenzae (NTHi) reside in 
      macrophage-like cells in human adenoid tissue. This study investigated the 
      ability of nonopsonized NTHi and encapsulated H. influenzae type b (Hib) to enter 
      human monocytic and epithelial cells. The number of intracellular bacteria was 
      determined by a viability assay and flow cytometry. To characterize the 
      mechanisms responsible for the internalization of NTHi, different inhibitors of 
      surface molecules, receptor turnover, and the cytoskeleton were used. Hib were 
      found in monocytic cells at very low numbers (<100 bacteria/2x105 cells). In 
      contrast, a great variation in intracellular numbers was detected between the 
      different NTHi isolates (range, 0.0007%-0.28% of the inoculum for monocytes and 
      0.053%-3.5% for epithelial cells). NTHi entered human monocytic and epithelial 
      cells via a receptor-mediated endocytosis involving mainly a beta-glucan receptor 
      that could be blocked by laminarin.
FAU - Ahren, I L
AU  - Ahren IL
AD  - Department of Medical Microbiology, Malmo University Hospital, Lund University, 
      Malmo, Sweden.
FAU - Williams, D L
AU  - Williams DL
FAU - Rice, P J
AU  - Rice PJ
FAU - Forsgren, A
AU  - Forsgren A
FAU - Riesbeck, K
AU  - Riesbeck K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010614
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (beta-glucan receptor)
RN  - 0 (platelet activating factor receptor)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Carcinoma/metabolism
MH  - Epithelial Cells/*metabolism
MH  - Flow Cytometry
MH  - Fluorescence
MH  - Haemophilus influenzae/*metabolism
MH  - Humans
MH  - Leukemia, Monocytic, Acute/metabolism
MH  - Lung Neoplasms/metabolism
MH  - Mannose/antagonists & inhibitors
MH  - Microscopy, Electron
MH  - Monocytes/*metabolism
MH  - Platelet Membrane Glycoproteins/antagonists & inhibitors
MH  - *Receptors, Cell Surface
MH  - *Receptors, G-Protein-Coupled
MH  - Receptors, Immunologic/antagonists & inhibitors/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 2001/06/26 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/06/26 10:00
PHST- 2000/06/02 00:00 [received]
PHST- 2001/04/18 00:00 [revised]
PHST- 2001/06/26 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/06/26 10:00 [entrez]
AID - JID000667 [pii]
AID - 10.1086/322016 [doi]
PST - ppublish
SO  - J Infect Dis. 2001 Jul 15;184(2):150-8. doi: 10.1086/322016. Epub 2001 Jun 14.