PMID- 11425255
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20190702
IS  - 0042-4900 (Print)
IS  - 0042-4900 (Linking)
VI  - 148
IP  - 22
DP  - 2001 Jun 2
TI  - Diagnostic investigation of chronic porcine reproductive and respiratory syndrome 
      virus in a breeding herd of pigs.
PG  - 687-90
AB  - Forty-five sows and 15 boars were selected at random from a breeding herd known 
      to be chronically infected with porcine reproductive and respiratory syndrome 
      virus (PRRSV) and lymphoid, immune-privileged, and 
      non-lymphoid/non-immune-privileged tissues were tested for the presence of the 
      virus by PCR, virus isolation, and immunohistochemistry. The virus was isolated 
      from the lateral retropharyngeal lymph node of one sow; the isolate was nucleic 
      acid sequenced and determined to be of field origin, and it was inoculated into 
      two PRRSV-naive pregnant sows (A and B) at 95 days of gestation. They were 
      necropsied 14 days later and samples of maternal and fetal tissue and blood 
      samples were collected. Sow A had 10 fresh, six partially autolysed, and two 
      mummified fetuses, and sow B had six fresh and viable fetuses. Viral nucleic acid 
      was detected by PCR in tissue pools from each sow and also from pooled fetal 
      tissues, and the virus was isolated from fetal pools from sow A.
FAU - Bierk, M D
AU  - Bierk MD
AD  - Department of Clinical and Population Sciences, University of Minnesota College 
      of Veterinary Medicine, St Paul 55108, USA.
FAU - Dee, S A
AU  - Dee SA
FAU - Rossow, K D
AU  - Rossow KD
FAU - Collins, J E
AU  - Collins JE
FAU - Guedes, M I
AU  - Guedes MI
FAU - Pijoan, C
AU  - Pijoan C
FAU - Molitor, T W
AU  - Molitor TW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
SB  - IM
EIN - Vet Rec 2001 Jul 21;149(3):90
MH  - Animals
MH  - Chronic Disease
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & 
      purification/pathogenicity
MH  - Swine
EDAT- 2001/06/27 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/06/27 10:00
PHST- 2001/06/27 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/06/27 10:00 [entrez]
AID - 10.1136/vr.148.22.687 [doi]
PST - ppublish
SO  - Vet Rec. 2001 Jun 2;148(22):687-90. doi: 10.1136/vr.148.22.687.