PMID- 11425892
OWN - NLM
STAT- MEDLINE
DCOM- 20010726
LR  - 20220309
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 21
IP  - 13
DP  - 2001 Jul 1
TI  - MIP-1alpha, MCP-1, GM-CSF, and TNF-alpha control the immune cell response that 
      mediates rapid phagocytosis of myelin from the adult mouse spinal cord.
PG  - 4649-56
AB  - The slow immune response in the adult mammalian CNS results in slow myelin 
      phagocytosis along degenerating white matter after injury. This has important 
      consequences for axon regeneration because of the presence of axon growth 
      inhibitors in myelin. In addition, abnormal immune cell responses in the CNS lead 
      to demyelinating disease. Lysophosphatidylcholine (LPC) can induce an 
      inflammatory response in the CNS, producing rapid demyelination without much 
      damage to adjacent cells. In this study, we searched for the molecular switches 
      that turn on this immune cell response. Using reverse transcription PCR analysis, 
      we show that mRNA expression of macrophage inflammatory protein-1alpha 
      (MIP-1alpha), macrophage chemotactic protein-1 (MCP-1), granulocyte 
      macrophage-colony stimulating factor (GM-CSF), and tumor necrosis factor-alpha 
      (TNF-alpha) in the spinal cord is rapidly and transiently upregulated after 
      intraspinal injection of LPC. Neutralizing these signaling molecules with 
      function-blocking antibodies suppresses recruitment of T-cells, neutrophils, and 
      monocytes into the spinal cord, as well as significantly reduces the number of 
      phagocytic macrophages and the demyelination induced by LPC. These findings will 
      have important implications for CNS regeneration and demyelinating disease.
FAU - Ousman, S S
AU  - Ousman SS
AD  - Centre for Research in Neuroscience, Montreal General Hospital Research Institute 
      and McGill University, Montreal, Quebec, Canada, H3G 1A4.
FAU - David, S
AU  - David S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Antibodies)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Cytokines)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antibodies/administration & dosage
MH  - Chemokine CCL2/antagonists & inhibitors/genetics/metabolism
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Cytokines/antagonists & inhibitors/genetics/*metabolism
MH  - Demyelinating Autoimmune Diseases, CNS/chemically induced/*immunology/pathology
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/antagonists & 
      inhibitors/genetics/metabolism
MH  - Lysophosphatidylcholines/pharmacology
MH  - Macrophage Activation/drug effects/immunology
MH  - Macrophage Inflammatory Proteins/antagonists & inhibitors/genetics/metabolism
MH  - Macrophages/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microinjections
MH  - Myelin Sheath/drug effects/*immunology
MH  - Neutrophil Infiltration/drug effects/immunology
MH  - Neutrophils/immunology
MH  - Phagocytosis/immunology
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spinal Cord/drug effects/*immunology/*metabolism/pathology
MH  - Spinal Cord Diseases/chemically induced/*immunology/pathology
MH  - T-Lymphocytes/immunology
MH  - Tumor Necrosis Factor-alpha/antagonists & inhibitors/genetics/metabolism
PMC - PMC6762369
EDAT- 2001/06/27 10:00
MHDA- 2001/07/28 10:01
PMCR- 2002/01/01
CRDT- 2001/06/27 10:00
PHST- 2001/06/27 10:00 [pubmed]
PHST- 2001/07/28 10:01 [medline]
PHST- 2001/06/27 10:00 [entrez]
PHST- 2002/01/01 00:00 [pmc-release]
AID - 21/13/4649 [pii]
AID - 5351 [pii]
AID - 10.1523/JNEUROSCI.21-13-04649.2001 [doi]
PST - ppublish
SO  - J Neurosci. 2001 Jul 1;21(13):4649-56. doi: 10.1523/JNEUROSCI.21-13-04649.2001.