PMID- 11426944
OWN - NLM
STAT- MEDLINE
DCOM- 20011214
LR  - 20181130
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 267
IP  - 2
DP  - 2001 Jul 15
TI  - HGF/SF induces mesothelial cell migration and proliferation by autocrine and 
      paracrine pathways.
PG  - 258-66
AB  - Mesothelial repair differs from that of other epithelial-like surfaces as healing 
      does not occur solely by centripetal in-growth of cells as a sheet from the wound 
      margins. Mesothelial cells lose their cell-cell junctions, divide, and adopt a 
      fibroblast-like morphology while scattering across and covering the wound 
      surface. These features are consistent with a cellular response to hepatocyte 
      growth factor/scatter factor (HGF/SF). In this study, we examined the ability of 
      mesothelial cells to secrete HGF/SF and investigated its possible role as an 
      autocrine regulator of mesothelial cell motility and proliferation. We found that 
      human primary mesothelial cells expressed HGF/SF mRNA and secreted active HGF/SF 
      into conditioned medium as determined by ELISA and in a scattering bioassay. 
      These cells also expressed the HGF/SF receptor, Met, as shown by RT-PCR and by 
      Western blot analysis and immunofluorescence. Incubation of mesothelial cells 
      with neutralizing antibodies to HGF/SF decreased cell migration to 25% of 
      controls, whereas addition of HGF/SF disrupted cell-cell junctions and induced 
      scattering and enhanced mesothelial cell migration. Furthermore, HGF/SF showed a 
      small but significant mitogenic effect on all mesothelial cell lines examined. In 
      conclusion, HGF/SF is produced by mesothelial cells and induces both motility and 
      proliferation of these cells. These data are consistent with HGF/SF playing an 
      autocrine role in mesothelial healing.
CI  - Copyright 2001 Academic Press.
FAU - Warn, R
AU  - Warn R
AD  - School of Biology, University of East Anglia, Norwich, NR4 7TJ, United Kingdom.
FAU - Harvey, P
AU  - Harvey P
FAU - Warn, A
AU  - Warn A
FAU - Foley-Comer, A
AU  - Foley-Comer A
FAU - Heldin, P
AU  - Heldin P
FAU - Versnel, M
AU  - Versnel M
FAU - Arakaki, N
AU  - Arakaki N
FAU - Daikuhara, Y
AU  - Daikuhara Y
FAU - Laurent, G J
AU  - Laurent GJ
FAU - Herrick, S E
AU  - Herrick SE
FAU - Mutsaers, S E
AU  - Mutsaers SE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Antineoplastic Agents)
RN  - 6032D45BEM (Suramin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Autocrine Communication/*physiology
MH  - Cell Division/physiology
MH  - Cell Movement/*physiology
MH  - Cells, Cultured
MH  - Epithelium/drug effects/metabolism/*physiology
MH  - Hepatocyte Growth Factor/genetics/*metabolism/pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Paracrine Communication/*physiology
MH  - Proto-Oncogene Proteins c-met/genetics/*metabolism
MH  - Suramin/pharmacology
EDAT- 2001/06/28 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/06/28 10:00
PHST- 2001/06/28 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/06/28 10:00 [entrez]
AID - S0014-4827(01)95240-1 [pii]
AID - 10.1006/excr.2001.5240 [doi]
PST - ppublish
SO  - Exp Cell Res. 2001 Jul 15;267(2):258-66. doi: 10.1006/excr.2001.5240.