PMID- 11427236
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1879-0909 (Electronic)
IS  - 0141-0229 (Linking)
VI  - 29
IP  - 1
DP  - 2001 Jul 5
TI  - Dechlorination of chlorophenols using extracellular peroxidases produced by 
      streptomyces albus ATCC 3005.
PG  - 62-69
AB  - Streptomyces albus ATCC 3005 was found to produce higher levels of extracellular 
      peroxidase activity (3.420 U mg(-1)) than previously reported for any other 
      actinomycete. Maximum peroxidase activity was obtained after 72 h of incubation 
      at a temperature of 30 degrees C in a liquid medium (pH 7.6) containing (in w/v) 
      0.8% to 0.9% oat spelts xylan and 0.6% yeast extract, corresponding to a C:N 
      ratio of around 8.4:1. Characterization of the peroxidases revealed that the 
      optimal temperature for peroxidase activity, using the standard 
      2,4-dichlorophenol (2,4-DCP) assay was 53 degrees C, when the enzyme reaction was 
      performed at pH 7.2. A study of the effect of temperature on the stability of 
      peroxidase over time, showed that the enzyme was stable at 40 degrees C, with a 
      half-life of 224 min, while at higher temperatures the stability and activity was 
      reduced such that at 50 degrees C and 70 degrees C the half-life of the enzyme 
      was 50 min and 9 min respectively. The optimum pH for the activity of the enzyme 
      occurred between pH 8.1 and 10.4. In terms of substrate specificity, the 
      peroxidase was able to catalyze a broad range of substrates including 2,4-DCP, 
      L-3,4-dihydroxyphenylalanine (L-DOPA), 2,4,5-trichlorophenol and other 
      chlorophenols in the presence of hydrogen peroxide. Ion exchange chromatography 
      was used to confirm that the enzyme was able to release chloride ions from a 
      range of chlorophenols.
FAU - Antonopoulos, V T
AU  - Antonopoulos VT
AD  - Department of Biological Sciences, John Tabor Laboratories, University of Essex, 
      Wivenhoe Park, CO4 3SQ, Colchester, United Kingdom
FAU - Rob, A
AU  - Rob A
FAU - Ball, A S
AU  - Ball AS
FAU - Wilson, M T
AU  - Wilson MT
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Enzyme Microb Technol
JT  - Enzyme and microbial technology
JID - 8003761
EDAT- 2001/06/28 10:00
MHDA- 2001/06/28 10:01
CRDT- 2001/06/28 10:00
PHST- 2001/06/28 10:00 [pubmed]
PHST- 2001/06/28 10:01 [medline]
PHST- 2001/06/28 10:00 [entrez]
AID - S014102290100357X [pii]
AID - 10.1016/s0141-0229(01)00357-x [doi]
PST - ppublish
SO  - Enzyme Microb Technol. 2001 Jul 5;29(1):62-69. doi: 
      10.1016/s0141-0229(01)00357-x.