PMID- 11429153
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20060712
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 7
IP  - 3
DP  - 2001 Jun
TI  - Establishment of heterotropic liver tissue mass with direct link to the host 
      liver following implantation of hepatocytes transfected with vascular endothelial 
      growth factor gene in mice.
PG  - 335-44
AB  - One of the major goals of tissue engineering is to establish an integrated organ 
      in vivo. We have previously shown that transfection of vascular endothelial 
      growth factor (VEGF) gene into hepatocytes promotes tissue formation by engrafted 
      cells. Here we show that tissue growth was significantly enhanced by 
      co-transplantation of hepatocyte growth factor (HGF) and tumor necrosis 
      factor-alpha (TNF alpha) gene transfected hepatocytes with VEGF-gene transfected 
      cells, but tissue islands were scattered nonspecifically in the abdomen of mice. 
      The result brought us forward to the next step to establish an integrated mass 
      and structural formation of liver tissue. We entrapped VEGF gene transfected 
      hepatocytes in a nylon mesh bag and intraperitoneally engrafted close to the 
      liver. Three weeks later, the bag was covered by a thick network of blood 
      vessels, compared to the control. Histological examination showed that the blood 
      vessels penetrated the parenchyma of the engrafted bag and formed a 
      well-developed vessel network in the region. The use of hepatocytes from lacZ 
      transgenic mice and PCR analysis demonstrated survival and albumin production by 
      hepatocytes in the engrafted bag. Our model can potentially be developed into a 
      heterotropic artificial liver with direct access to the host blood circulation.
FAU - Ajioka, I
AU  - Ajioka I
AD  - Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama 
      226-8501, Japan.
FAU - Nishio, R
AU  - Nishio R
FAU - Ikekita, M
AU  - Ikekita M
FAU - Akaike, T
AU  - Akaike T
FAU - Sasaki, M
AU  - Sasaki M
FAU - Enami, J
AU  - Enami J
FAU - Watanabe, Y
AU  - Watanabe Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
RN  - 0 (Actins)
RN  - 0 (Albumins)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Actins/biosynthesis
MH  - Albumins/biosynthesis
MH  - Animals
MH  - Biocompatible Materials/pharmacology
MH  - Biomedical Engineering
MH  - Cell Survival/drug effects
MH  - Coated Materials, Biocompatible/chemistry
MH  - Endothelial Growth Factors/*genetics/*physiology
MH  - Female
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/genetics/pharmacology
MH  - Hepatocytes/*transplantation
MH  - Humans
MH  - Lac Operon/physiology
MH  - Liver/*blood supply/*physiology
MH  - Lymphokines/*genetics/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Neovascularization, Physiologic/drug effects
MH  - Recombinant Proteins
MH  - Surface Properties
MH  - Transfection
MH  - Transplantation, Heterotopic/methods
MH  - Tumor Necrosis Factor-alpha/genetics/pharmacology
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2001/06/29 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/06/29 10:00
PHST- 2001/06/29 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/06/29 10:00 [entrez]
AID - 10.1089/10763270152044198 [doi]
PST - ppublish
SO  - Tissue Eng. 2001 Jun;7(3):335-44. doi: 10.1089/10763270152044198.