PMID- 11429160
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20181130
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 21
IP  - 5
DP  - 2001 May
TI  - Monocyte chemoattractant protein-1 expression by osteoblasts following infection 
      with Staphylococcus aureus or Salmonella.
PG  - 297-304
AB  - Two common pathogens of bone, Staphylococcus aureus and Salmonella, were 
      investigated for their ability to induce chemokine expression in bone-forming 
      osteoblasts. Cultured mouse or human osteoblasts could rapidly respond to 
      bacterial infection by upregulating the mRNA encoding the chemokine, monocyte 
      chemoattractant protein-1 (MCP-1). This rapid induction occurred on infection 
      with either the gram-positive pathogen, S. aureus, or the gram-negative pathogen, 
      Salmonella. Increased mRNA expression translated into MCP-1 secretion by cultured 
      mouse or human osteoblasts in response to viable bacteria, whereas UV-killed 
      bacteria were less effective in stimulating chemokine secretion. There was a 
      dose-response relationship observed between the amount of input bacteria and 
      increases in MCP-1 secretion. Immunohistochemical staining of infected 
      osteoblasts indicated that the majority of cells could express MCP-1, with some 
      osteoblasts having a higher intensity of staining than others. Organ cultures of 
      mouse calvaria (skullcap) bone showed increases in MCP-1 immunostaining following 
      bacterial infection. The immunoreactive MCP-1 in infected calvaria localized to 
      areas containing active osteoblasts. Taken together, these studies demonstrate a 
      conserved osteoblast-derived MCP-1 response to two very different pathogens of 
      bone.
FAU - Bost, K L
AU  - Bost KL
AD  - Department of Biology, University of North Carolina at Charlotte, 9201 University 
      City Boulevard, Charlotte, NC 28223-0001, USA. klbost@email.unc.edu
FAU - Bento, J L
AU  - Bento JL
FAU - Petty, C C
AU  - Petty CC
FAU - Schrum, L W
AU  - Schrum LW
FAU - Hudson, M C
AU  - Hudson MC
FAU - Marriott, I
AU  - Marriott I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Organ Culture Techniques
MH  - Osteoblasts/*metabolism/*microbiology
MH  - RNA, Messenger/biosynthesis/metabolism
MH  - Salmonella enterica/*immunology
MH  - Staphylococcus aureus/*immunology
EDAT- 2001/06/29 10:00
MHDA- 2001/08/24 10:01
CRDT- 2001/06/29 10:00
PHST- 2001/06/29 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/06/29 10:00 [entrez]
AID - 10.1089/107999001300177484 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2001 May;21(5):297-304. doi: 
      10.1089/107999001300177484.