PMID- 11429269
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20191104
IS  - 0891-0618 (Print)
IS  - 0891-0618 (Linking)
VI  - 21
IP  - 4
DP  - 2001 Jun
TI  - Depletion of glial cell line-derived neurotrophic factor in substantia nigra 
      neurons of Parkinson's disease brain.
PG  - 277-88
AB  - The distribution of nerve growth factor (NGF), ciliary neurotrophic factor 
      (CNTF), glial cell line-derived neurotrophic factor (GDNF), brain derived 
      neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) in 
      substantia nigra pars compacta (SNc) of Parkinson's disease (PD) brains was 
      investigated by immunofluorescence. Cases studied included four 69-77 year old 
      neurologically normal male controls and four 72-79 year old male PD patients. 
      Integrated optical densities (IODs) of immunofluorescence over individual 
      neuromelanin-containing neurons and in areas of neuropil and the number of 
      neurons on H & E stained adjacent sections were quantitated with the use of the 
      BioQuant Image Analyzer. Data were statistically analyzed by ANOVA, including the 
      unpaired two-tailed Student t-test and the Mann-Whitney test. The results showed 
      55.8% (P<0.0001) dropout of SNc neurons in PD brains compared to age-matched 
      controls. Despite considerable neuronal dropout, immunofluorescent NTFs in the PD 
      brains showed differential reductions that were consistent within the group as 
      compared to age-matched controls: reductions were GDNF, 19.4%/neuron (P<0.0001), 
      20.2%/neuropil (P<0.0001); CNTF, 11.1%/neuron (P<0.0001), 9.4%/neuropil 
      (P<0.0001); BDNF, 8.6%/neuron (P<0.0001), 2.5%/neuropil. NGF, NT-3 and NT-4 
      showed no significant differences within surviving neurons or neuropil. Since the 
      depletion of GDNF both within surviving neurons and neuropil was twice as great 
      as that of CNTF and BDNF and since the other NTFs showed no changes, GDNF, of the 
      tested NTFs, is probably the most susceptible and the earliest to decrease in the 
      surviving neurons of SNc. These observations suggest a role for decreased 
      availability of GDNF in the process of SNc neurodegeneration in PD.
FAU - Chauhan, N B
AU  - Chauhan NB
AD  - Research and Development Service, Edward Hines, Jr., Veterans Affairs Hospital, 
      Hines, IL 60141, USA.
FAU - Siegel, G J
AU  - Siegel GJ
FAU - Lee, J M
AU  - Lee JM
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chem Neuroanat
JT  - Journal of chemical neuroanatomy
JID - 8902615
RN  - 0 (GDNF protein, human)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Aged
MH  - Blotting, Western
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Male
MH  - Mesencephalon/metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - Parkinson Disease/*metabolism/pathology
MH  - Substantia Nigra/*cytology/*metabolism
EDAT- 2001/06/29 10:00
MHDA- 2001/08/24 10:01
CRDT- 2001/06/29 10:00
PHST- 2001/06/29 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/06/29 10:00 [entrez]
AID - S0891061801001156 [pii]
AID - 10.1016/s0891-0618(01)00115-6 [doi]
PST - ppublish
SO  - J Chem Neuroanat. 2001 Jun;21(4):277-88. doi: 10.1016/s0891-0618(01)00115-6.