PMID- 11431013
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20190826
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 117
IP  - 1-2
DP  - 2001 Jul 2
TI  - Identification of dopamine plasma membrane and vesicular transporters in human 
      peripheral blood lymphocytes.
PG  - 133-42
AB  - Plasma membrane dopamine transporter (DAT), vesicular monoamine transporters 
      (VMAT) type-1 and -2 and the expression of the dopaminergic markers dopamine and 
      tyrosine hydroxylase were assessed in membranes and/or in cytospin centrifuged 
      human peripheral blood lymphocytes. The radiolabeled DAT ligand [3H]GBR12935 was 
      bound to peripheral lymphocytes in a manner consistent with the specific binding 
      to a dopamine uptake system, with a dissociation constant similar to that found 
      in striatum, but with a lower density of binding sites. On the other hand, no 
      specific binding occurred in cerebellum used as a test tissue not expressing DAT. 
      Western blot analysis using antibodies raised against amino or carboxy terminus 
      of DAT or against VMAT-1 or VMAT-2 revealed labeling of single bands of 
      approximately 76, 55 or 68 KDa, respectively, displaying similar migration 
      characteristics in lymphocytes and test tissues used for comparison. 
      Immunofluorescence revealed that anti-dopamine, anti-tyrosine hydroxylase, 
      anti-DAT, anti-VMAT-1 and anti-VMAT-2 antibodies labeled the total population of 
      cytospin-centrifuged lymphocytes mounted on microscope slides. Confocal laser 
      microscopy demonstrated that dopamine and VMAT-2 immunoreactivity was developed 
      mainly in cytoplasmic punctiform areas likely corresponding to vesicles and to a 
      lower extent was associated to plasma membrane. Tyrosine hydroxylase 
      immunoreactivity was diffused to cytoplasm and to plasma membrane of lymphocytes, 
      whereas DAT and VMAT-1 immunoreactivity were located almost exclusively in 
      lymphocyte plasma membrane and cytoplasm, respectively. Lymphocyte DAT 
      characterized in this study has probably functional relevance as [3H]dopamine was 
      taken up by intact lymphocytes and uptake was inhibited specifically by compounds 
      known to affect dopamine transport. These findings indicate that human peripheral 
      blood lymphocytes possess DAT plasma membrane and VMAT-1 and VMAT-2 transporters. 
      Increasing evidence indicates that dopamine transporter changes may be related to 
      neuronal injury. In view of this assessment of lymphocyte DAT and VMAT 
      transporters can be considered for identifying pathologies characterized by 
      impaired dopaminergic neurotransmission.
FAU - Amenta, F
AU  - Amenta F
AD  - Sezione di Anatomia Umana, Dipartimento di Scienze Farmacologiche e Medicina 
      Sperimentale, Universita di Camerino, Via Scalzino, 3, 62032, Camerino, Italy. 
      amenta@cambio.unicam.it
FAU - Bronzetti, E
AU  - Bronzetti E
FAU - Cantalamessa, F
AU  - Cantalamessa F
FAU - El-Assouad, D
AU  - El-Assouad D
FAU - Felici, L
AU  - Felici L
FAU - Ricci, A
AU  - Ricci A
FAU - Tayebati, S K
AU  - Tayebati SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Carrier Proteins)
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (SLC18A1 protein, human)
RN  - 0 (SLC18A2 protein, human)
RN  - 0 (SLC6A3 protein, human)
RN  - 0 (Vesicular Biogenic Amine Transport Proteins)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Adult
MH  - Carrier Proteins/*analysis
MH  - Cell Membrane/*chemistry
MH  - Dopamine/metabolism
MH  - Dopamine Plasma Membrane Transport Proteins
MH  - Humans
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Lymphocytes/*chemistry
MH  - Male
MH  - Membrane Glycoproteins/*analysis
MH  - *Membrane Transport Proteins
MH  - Middle Aged
MH  - *Nerve Tissue Proteins
MH  - *Neuropeptides
MH  - Radioligand Assay
MH  - Vesicular Biogenic Amine Transport Proteins
MH  - Vesicular Monoamine Transport Proteins
EDAT- 2001/06/30 10:00
MHDA- 2001/08/24 10:01
CRDT- 2001/06/30 10:00
PHST- 2001/06/30 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/06/30 10:00 [entrez]
AID - S0165572801003174 [pii]
AID - 10.1016/s0165-5728(01)00317-4 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2001 Jul 2;117(1-2):133-42. doi: 10.1016/s0165-5728(01)00317-4.