PMID- 11432792
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 130
IP  - 1
DP  - 2001 Jul
TI  - Analysis of tyrosine phosphorylation-dependent protein-protein interactions in 
      TrkB-mediated intracellular signaling using modified yeast two-hybrid system.
PG  - 157-65
AB  - Activated receptor tyrosine kinases induce a large number of tyrosine 
      phosphorylation-dependent protein-protein interactions through which they mediate 
      their various ligand-exerted functions including regulation of proliferation, 
      differentiation and survival. TrkB receptor tyrosine kinase activated by binding 
      of brain-derived neurotrophic factor (BDNF) also stimulates various protein 
      interactions in a tyrosine phosphorylation-dependent manner in neuronal cells. To 
      examine tyrosine phosphorylation-dependent interactions stimulated by active 
      TrkB, we developed a modified yeast two-hybrid system, which we call the yeast 
      two-and-a-half-hybrid system. In this system, yeast was engineered to express a 
      tyrosine kinase domain of TrkB as an effector, in addition to two fusion proteins 
      with GAL4 DNA-binding and GAL4 activation domains as bait and prey proteins, 
      respectively. Using this system with Shp2 as the bait, we demonstrated that Shp2 
      interacts directly with BIT/SHPS-1 (also called SIRP) and Grb2 depending on 
      tyrosine phosphorylation mediated by TrkB. Furthermore, we screened an adult 
      human brain cDNA library with the yeast two-and-a-half-hybrid system in order to 
      identify other Shp2-binding proteins in TrkB-stimulated tyrosine phosphorylation 
      signaling. We found that fibroblast growth factor receptor substrate 2beta 
      (FRS2beta), also called SNT2, interacts with Shp2 dependently on TrkB-mediated 
      tyrosine phosphorylation of FRS2beta/SNT2. Therefore, we show that the 
      two-and-a-half-hybrid system is a powerful tool for studying tyrosine 
      phosphorylation-dependent protein-protein interactions in intracellular signaling 
      pathways stimulated by TrkB receptor tyrosine kinase.
FAU - Yamada, M
AU  - Yamada M
AD  - Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 
      565-0871, Japan. yamada@protein.osaka-u.ac.jp
FAU - Suzuki, K
AU  - Suzuki K
FAU - Mizutani, M
AU  - Mizutani M
FAU - Asada, A
AU  - Asada A
FAU - Matozaki, T
AU  - Matozaki T
FAU - Ikeuchi, T
AU  - Ikeuchi T
FAU - Koizumi, S
AU  - Koizumi S
FAU - Hatanaka, H
AU  - Hatanaka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Carrier Proteins)
RN  - 0 (FRS2 protein, human)
RN  - 0 (FRS3 protein, human)
RN  - 0 (GRB2 Adaptor Protein)
RN  - 0 (GRB2 protein, human)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Lipoproteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neural Cell Adhesion Molecule L1)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.1.3.48 (PTPN11 protein, human)
RN  - EC 3.1.3.48 (PTPN6 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 6)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Adult
MH  - *Antigens, Differentiation
MH  - Brain/metabolism
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Line
MH  - Electrophoresis, Polyacrylamide Gel
MH  - GRB2 Adaptor Protein
MH  - Humans
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Lipoproteins/genetics/*metabolism
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Membrane Proteins/genetics/*metabolism
MH  - *Neural Cell Adhesion Molecule L1
MH  - Neural Cell Adhesion Molecules/genetics/*metabolism
MH  - Phosphoproteins/genetics/*metabolism
MH  - Phosphorylation
MH  - Phosphotyrosine/metabolism
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 6
MH  - Protein Tyrosine Phosphatases/genetics/*metabolism
MH  - Proteins/genetics/*metabolism
MH  - Receptor, trkB/chemistry/genetics/*metabolism
MH  - *Receptors, Immunologic
MH  - Signal Transduction
MH  - Two-Hybrid System Techniques
EDAT- 2001/07/04 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/07/04 10:00
PHST- 2001/07/04 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/07/04 10:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a002955 [doi]
PST - ppublish
SO  - J Biochem. 2001 Jul;130(1):157-65. doi: 10.1093/oxfordjournals.jbchem.a002955.