PMID- 11434972
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20191210
IS  - 0168-1656 (Print)
IS  - 0168-1656 (Linking)
VI  - 88
IP  - 3
DP  - 2001 Jul 12
TI  - Improvement of a mediator-type biochemical oxygen demand sensor for on-site 
      measurement.
PG  - 269-75
AB  - We characterized a mediator-type biochemical oxygen demand (BOD) sensor with a 
      three-electrode system using potassium ferricyanide (FC) and Pseudomonas 
      fluorescens in our previous study. In the present study, we have utilized the 
      advantages of a mediator-type biosensor, which does not require air-supply 
      equipment for on-site measurements, and made a fully disposable sensor tip for a 
      portable device. The tip consists of a two-electrode system with P. fluorescens 
      immobilized on a cellulose acetate membrane and is packaged in polyester film to 
      prevent it from drying out. By aeration with a 0.1 M NaCl solution of P. 
      fluorescens (after growth), the sensor responses as well as their reproducibility 
      and stability have been successfully improved. The responses increased more than 
      seven times, and the calibration curve from 15 to 260 mg l(-1) also remained 
      linear although the response decreased approximately half the original after at 
      least 35 days in storage. The reproducibility of the sensor responses improved to 
      12.7% (average of relative standard deviations (RSDs)) in the calibration curve 
      obtained by using the Organization for Economic Cooperation and Development 
      synthetic sewage. Examination of real samples from three different sources showed 
      that the BOD as determined by the sensor correlates well with the conventional 
      5-day BOD method (r(2)=0.982, 0.823, and 0.809). Consequently, the aeration 
      process makes it possible to realize rapid, and in situ measurements without the 
      long conditioning process that is generally required to activate the 
      microorganisms immobilized on bio-films before use. Finally, we have designed a 
      portable device that utilizes our disposable sensor tip.
FAU - Yoshida, N
AU  - Yoshida N
AD  - Akebono Research and Development Center, Ltd., 6-1-12 Midoricho, Kasukabe-si, 
      344-0063, Saitama, Japan. karube@bio.rcast.u-tokyo.ac.jp
FAU - Hoashi, J
AU  - Hoashi J
FAU - Morita, T
AU  - Morita T
FAU - McNiven, S J
AU  - McNiven SJ
FAU - Nakamura, H
AU  - Nakamura H
FAU - Karube, I
AU  - Karube I
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (Sewage)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - *Biosensing Techniques/economics/instrumentation/methods
MH  - Biotechnology
MH  - Calibration
MH  - Environmental Monitoring
MH  - Oxygen/*analysis
MH  - Pseudomonas fluorescens/*metabolism
MH  - Reproducibility of Results
MH  - Sewage
EDAT- 2001/07/04 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/07/04 10:00
PHST- 2001/07/04 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/07/04 10:00 [entrez]
AID - S0168165601002826 [pii]
AID - 10.1016/s0168-1656(01)00282-6 [doi]
PST - ppublish
SO  - J Biotechnol. 2001 Jul 12;88(3):269-75. doi: 10.1016/s0168-1656(01)00282-6.