PMID- 11435698
OWN - NLM
STAT- MEDLINE
DCOM- 20010802
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 92
IP  - 3-4
DP  - 2001
TI  - Molecular cytogenetic analysis of the highly repetitive DNA in the genome of 
      Apodemus argenteus, with comments on the phylogenetic relationships in the genus 
      Apodemus.
PG  - 254-63
AB  - The DNA of Apodemus argenteus was digested with DraI, and the resultant DraI 
      fragment of highly repetitive DNA was isolated and analyzed by DNA filter 
      hybridization, cloning, sequencing, and fluorescence in situ hybridization 
      (FISH). Southern blot hybridization and nucleotide sequencing revealed that most 
      of the DraI fragment consisted of a 230-bp repeating unit and contained no 
      sex-chromosome-specific nucleotide sequences. The DraI fragment included the 
      CENP-B box-like sequence, with a strong homology to the human CENP-B box 
      sequence. FISH revealed that the DraI fragment was specific to all 
      pericentromeric C-band-positive regions, as well as to the C-block of the X 
      chromosome. No hybridization signals were obtained from A. speciosus, A. 
      peninsulae peninsulae, A.p. giliacus, A. agrarius, A. sylvaticus, A. semotus, or 
      Mus musculus when the DraI fragment was used as probe. Peptide nucleic acid 
      (PNA)-FISH using the CENP-B box-like sequence in the DraI fragments as probe 
      suggested that this nucleotide sequence was also specific to all pericentromeric 
      C-heterochromatic regions of A. argenteus chromosomes. Zoo-blot hybridization 
      using DraI-digested genomic DNA from three species of Apodemus (namely, A. 
      argenteus, A. speciosus, and A. peninsulae) and from Mus musculus strongly 
      suggested that the consensus DraI fragment contained nucleotide sequences that 
      were species-specific for A. argenteus. These results also suggest that A. 
      argenteus is phylogenetically distant from other Apodemus species examined, as 
      well as the possibility that the DraI fragment might be related directly to the 
      delayed quinacrine mustard fluorescence of many pericentromeric C-heterochromatic 
      regions of the chromosomes in A. argenteus.
CI  - Copyright 2001 S. Karger AG, Basel.
FAU - Fukushi, D
AU  - Fukushi D
AD  - Department of Biofunctional Science, Faculty of Agriculture and Life Science, 
      Hirosaki University, Hirosaki, Japan.
FAU - Kuro-O, M
AU  - Kuro-O M
FAU - Shichiri, M
AU  - Shichiri M
FAU - Obara, Y
AU  - Obara Y
FAU - Tsuchiya, K
AU  - Tsuchiya K
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Autoantigens)
RN  - 0 (Cenpb protein, mouse)
RN  - 0 (Centromere Protein B)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heterochromatin)
RN  - 0 (Peptide Nucleic Acids)
RN  - 4213-45-0 (Quinacrine Mustard)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (TTTAAA -specific type II deoxyribonucleases)
SB  - IM
MH  - Animals
MH  - *Autoantigens
MH  - Base Sequence
MH  - Binding Sites
MH  - Blotting, Southern
MH  - Centromere Protein B
MH  - Chromosomal Proteins, Non-Histone/metabolism
MH  - Chromosomes/*genetics
MH  - Cloning, Molecular
MH  - Consensus Sequence/genetics
MH  - *DNA-Binding Proteins
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Electrophoresis, Agar Gel
MH  - Female
MH  - *Genome
MH  - Heterochromatin/genetics
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Muridae/*genetics
MH  - Peptide Nucleic Acids/genetics
MH  - *Phylogeny
MH  - Quinacrine Mustard
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Sex Chromosomes/genetics
MH  - Species Specificity
MH  - Staining and Labeling
EDAT- 2001/07/04 10:00
MHDA- 2001/08/03 10:01
CRDT- 2001/07/04 10:00
PHST- 2001/07/04 10:00 [pubmed]
PHST- 2001/08/03 10:01 [medline]
PHST- 2001/07/04 10:00 [entrez]
AID - 56913 [pii]
AID - 10.1159/000056913 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 2001;92(3-4):254-63. doi: 10.1159/000056913.