PMID- 11436011
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20061115
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 130
IP  - 1
DP  - 2001 Jul
TI  - The induction of angiogenesis by the implantation of autologous bone marrow 
      cells: a novel and simple therapeutic method.
PG  - 44-54
AB  - BACKGROUND: Bone marrow contains many kinds of primitive cells that could 
      differentiate to endothelial cells and secrete several growth factors. In the 
      current study, we attempted to induce therapeutic angiogenesis by implanting 
      autologous bone marrow cells (BMCs) and using a rat ischemic hind limb model. 
      METHODS: BMCs were prepared by removing red blood cells. A rat ischemic hind limb 
      model was made by the ligation of the left femoral artery and its branches. BMCs 
      were injected into 7 points of the ischemic muscles. To assess angiogenesis, a 
      microangiogram, laser Doppler, and histologic evaluation were performed after the 
      surgical procedure. RESULTS: A microangiogram and histologic evaluation showed 
      that angiogenesis was significantly induced in the ischemic hind limb by the 
      implantation of BMCs. Laser Doppler imaging analysis showed that blood flow was 
      significantly increased after implantation of BMCs. Some implanted BMCs were 
      stained positively with CD31 and vascular endothelial-cadherin (VE-cadherin), 
      which might have been incorporated into the vasculature. The condition of 
      ischemia caused an elevation in the level of basic fibroblast growth factor in 
      the ischemic muscle and also in interleukin-1beta derived from the implanted 
      BMCs, which might contribute to angiogenesis. CONCLUSION: These findings indicate 
      that autologous bone marrow implantation may be a novel and simple method for 
      inducing therapeutic angiogenesis.
FAU - Hamano, K
AU  - Hamano K
AD  - First Department of Surgery, Yamaguchi University School of Medicine, Yamaguchi, 
      Japan.
FAU - Li, T S
AU  - Li TS
FAU - Kobayashi, T
AU  - Kobayashi T
FAU - Tanaka, N
AU  - Tanaka N
FAU - Kobayashi, S
AU  - Kobayashi S
FAU - Matsuzaki, M
AU  - Matsuzaki M
FAU - Esato, K
AU  - Esato K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
RN  - 0 (Cytokines)
RN  - 0 (Growth Substances)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism/physiology
MH  - *Bone Marrow Transplantation
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Endothelium, Vascular/cytology
MH  - Growth Substances/metabolism
MH  - Hindlimb/blood supply
MH  - Ischemia/pathology/physiopathology
MH  - Muscle, Skeletal/blood supply/pathology/physiopathology
MH  - Neovascularization, Physiologic/*physiology
MH  - Rats
MH  - Regional Blood Flow
MH  - Transplantation, Autologous
EDAT- 2001/07/04 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/07/04 10:00
PHST- 2001/07/04 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/07/04 10:00 [entrez]
AID - S0039-6060(01)59489-1 [pii]
AID - 10.1067/msy.2001.114762 [doi]
PST - ppublish
SO  - Surgery. 2001 Jul;130(1):44-54. doi: 10.1067/msy.2001.114762.