PMID- 11437499
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20031114
IS  - 0021-9975 (Print)
IS  - 0021-9975 (Linking)
VI  - 124
IP  - 4
DP  - 2001 May
TI  - Detection of European porcine reproductive and respiratory syndrome virus in 
      porcine alveolar macrophages by two-colour immunofluorescence and in-situ 
      hybridization-immunohistochemistry double labelling.
PG  - 238-45
AB  - Two groups of five pigs aged 6 weeks were each infected oronasally with one of 
      two different European isolates of porcine reproductive and respiratory syndrome 
      virus (PRRSV). The animals were killed sequentially at 4, 7, 14 or 21 days 
      post-inoculation for examination. The methods used consisted of histopathology, 
      and mono- and double-labelling techniques based on in-situ hybridization, 
      immunofluorescence and immunohistochemistry. Porcine alveolar macrophages (PAMs) 
      contained large amounts of PRRSV antigen and PRRSV RNA, as shown by double 
      labelling with (1) either PRRSV immunofluorescence or PRRSV-specific in-situ 
      hybridization with digoxigenin-labelled riboprobes, and (2) immunolabelling with 
      Mac 387 antibody for calprotectin. Expression of PRRSV-RNA was not detectable in 
      cytokeratin-positive hypertrophic and proliferating pneumocytes or in cells of 
      alveolar ducts or bronchiolar epithelium. The use of two-colour 
      immunofluorescence with confocal laser scanning microscopy and double labelling 
      with in-situ hybridization-immunohistochemistry showed that PAMs were the only 
      pulmonary target cells. This contradicts earlier reports that epithelial 
      pulmonary cells may also be infected by PRRSV.
FAU - Teifke, J P
AU  - Teifke JP
AD  - Institut fur Infektionsmedizin, Bundesforschungsanstalt fur Viruskrankheitein der 
      Tiere, Insel Riems, Germany.
FAU - Dauber, M
AU  - Dauber M
FAU - Fichtner, D
AU  - Fichtner D
FAU - Lenk, M
AU  - Lenk M
FAU - Polster, U
AU  - Polster U
FAU - Weiland, E
AU  - Weiland E
FAU - Beyer, J
AU  - Beyer J
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Female
MH  - Fluorescent Antibody Technique/methods/*veterinary
MH  - Immunohistochemistry/methods/*veterinary
MH  - In Situ Hybridization/methods/*veterinary
MH  - Lung/pathology/virology
MH  - Macrophages, Alveolar/pathology/*virology
MH  - Microscopy, Confocal/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/transmission/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/*isolation & purification
MH  - Pregnancy
MH  - RNA, Viral/analysis
MH  - *Swine
EDAT- 2001/07/05 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/07/05 10:00
PHST- 2001/07/05 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/07/05 10:00 [entrez]
AID - S0021-9975(00)90458-4 [pii]
AID - 10.1053/jcpa.2000.0458 [doi]
PST - ppublish
SO  - J Comp Pathol. 2001 May;124(4):238-45. doi: 10.1053/jcpa.2000.0458.