PMID- 11437506
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20061115
IS  - 0021-9975 (Print)
IS  - 0021-9975 (Linking)
VI  - 124
IP  - 4
DP  - 2001 May
TI  - Comparative study of in-situ cell death induced by the viruses of viral 
      haemorrhagic septicaemia (VHS) and infectious pancreatic necrosis (IPN) in 
      rainbow trout.
PG  - 300-7
AB  - The causative viruses of two diseases of rainbow trout, viral haemorrhagic 
      septicaemia (VHS) and infectious pancreatic necrosis (IPN), exert much of their 
      cytopathogenic effect in cell culture through the induction of apoptosis. In the 
      present study, the TUNEL procedure was used to investigate the presence of 
      apoptotic cells in different organs of rainbow trout infected with the viruses of 
      VHS and IPN. VHS viral infection resulted in massive apoptosis in renal lymphoid 
      tissue, where viral antigens were also detected. Large numbers of viral particles 
      were observed in close proximity to apoptotic cells. Apoptosis was not detected 
      in excretory cells of the renal tubules or in infected muscle cells. IPN virus 
      did not induce apoptosis in the pancreas. However, the DNA degradation associated 
      with apoptotic nuclei was observed in muscle lesions. Taken together, these 
      results indicated that induction of apoptosis in vivo was critically influenced 
      by the species of virus and the cell type. Moreover, it would seem likely that 
      apoptosis contributed to the nature of the two diseases and to mortality.
FAU - Eleouet, J F
AU  - Eleouet JF
AD  - UnitAe de Virologie et Immunologie Moleculaires, Institut National de la 
      Recherche Agronomique, Jouy-en-Josas, 78352, France.
FAU - Druesne, N
AU  - Druesne N
FAU - Chilmonczyk, S
AU  - Chilmonczyk S
FAU - Monge, D
AU  - Monge D
FAU - Dorson, M
AU  - Dorson M
FAU - Delmas, B
AU  - Delmas B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - *Apoptosis
MH  - Birnaviridae Infections/pathology/*veterinary
MH  - DNA Fragmentation
MH  - Fish Diseases/*pathology/virology
MH  - Fluorescent Antibody Technique, Indirect/veterinary
MH  - In Situ Nick-End Labeling/veterinary
MH  - Infectious pancreatic necrosis virus/isolation & 
      purification/*physiology/ultrastructure
MH  - Kidney Tubules/pathology/virology
MH  - Microscopy, Fluorescence/veterinary
MH  - Muscle, Skeletal/microbiology/pathology
MH  - Myocardium/ultrastructure
MH  - Oncorhynchus mykiss/*virology
MH  - Pancreatic Diseases/pathology/*veterinary/virology
MH  - Rhabdoviridae/isolation & purification/*physiology
MH  - Rhabdoviridae Infections/pathology/*veterinary
MH  - Species Specificity
EDAT- 2001/07/05 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/07/05 10:00
PHST- 2001/07/05 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/07/05 10:00 [entrez]
AID - S0021-9975(01)90467-0 [pii]
AID - 10.1053/jcpa.2001.0467 [doi]
PST - ppublish
SO  - J Comp Pathol. 2001 May;124(4):300-7. doi: 10.1053/jcpa.2001.0467.