PMID- 11440148
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20091119
IS  - 0916-8451 (Print)
IS  - 0916-8451 (Linking)
VI  - 65
IP  - 5
DP  - 2001 May
TI  - Visualization of biallelic expression of the imprinted SNRPN gene induced by 
      inhibitors of DNA methylation and histone deacetylation.
PG  - 1236-9
AB  - We have adapted a fluorescence in situ hybridization (FISH) method to detect 
      nascent RNA molecules of the imprinted SNRPN gene at the initial transcription 
      sites in nuclei of human HL60 and WI38 cells. Simultaneous detection of RNA and 
      DNA of SNRPN by FISH using the cosmid probe confirmed its monoallelic expression 
      in these cell lines. Treatment of both cells by inhibitors of DNA methylation and 
      histone deacetylation resulted in time-dependent increase of the cell population 
      with the biallelic expression of SNRPN.
FAU - Kohda, A
AU  - Kohda A
AD  - Laboratory of Molecular and Cellular Biology, Faculty of Bioresources, Mie 
      University, Tsu, Japan.
FAU - Taguchi, H
AU  - Taguchi H
FAU - Okumura, K
AU  - Okumura K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Autoantigens)
RN  - 0 (Histones)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
RN  - 0 (SNRPN protein, human)
RN  - 0 (snRNP Core Proteins)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Acetylation
MH  - *Alleles
MH  - Autoantigens/*genetics
MH  - *DNA Methylation
MH  - Gene Expression Regulation/*drug effects
MH  - *Genomic Imprinting
MH  - HL-60 Cells
MH  - Histone Deacetylases/metabolism
MH  - Histones/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Ribonucleoproteins, Small Nuclear
MH  - snRNP Core Proteins
EDAT- 2001/07/07 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/07/07 10:00
PHST- 2001/07/07 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/07/07 10:00 [entrez]
AID - 10.1271/bbb.65.1236 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2001 May;65(5):1236-9. doi: 10.1271/bbb.65.1236.