PMID- 11440643
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20190610
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 21
IP  - 6
DP  - 2001 Jun
TI  - Signal transducers and activators of transcription (Stat) are detectable in mouse 
      trigeminal ganglion neurons.
PG  - 445-50
AB  - We studied signal transducers and activators of transcription (Stat) expression 
      in mouse trigeminal ganglia (TG) to gain an understanding of herpes simplex virus 
      (HSV) infection and reactivation. Mouse TG were harvested and were either frozen 
      for Western blot analysis or preserved in 4% paraformaldehyde for subsequent 
      immunohistochemistry study. The thawed specimens were homogenized, and 
      nuclear/cytoplasmic extractions were performed for Western blots and 
      immunoprecipitation. Immunohistochemistry showed that Stat1, Stat3, Stat4, 
      Stat5b, and phosphotyrosine Stat3 localized to TG neurons, not surrounding 
      satellite cells. Western blot of TG nuclear and cytoplasmic extracts confirmed 
      the presence of these Stat at the appropriate molecular weights. Stat2 was 
      undetectable in TG by these methods. Immunoprecipitation of TG nuclear extracts 
      did not confirm the presence of Stat-Stat dimers in these specimens. These 
      studies show that several Stat, including phosphotyrosine Stat3, are present in 
      TG neurons, the site of HSV latency, where they could act upon latent viral DNA 
      to effect reactivation.
FAU - Kriesel, J D
AU  - Kriesel JD
AD  - Department of Ophthalmology, The John A. Moran Eye Center, University of Utah 
      School of Medicine, Salt Lake City, UT 84132, USA. jkriesel@med.utah.edu
FAU - Jones, B B
AU  - Jones BB
FAU - Hwang, I P
AU  - Hwang IP
FAU - Dahms, K M
AU  - Dahms KM
FAU - Spruance, S L
AU  - Spruance SL
LA  - eng
GR  - EY11732/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Cytokines)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - Animals
MH  - Cytokines/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Herpes Simplex/metabolism/virology
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Biological
MH  - Neurons/metabolism
MH  - Signal Transduction
MH  - Trans-Activators/*metabolism
MH  - Trigeminal Ganglion/*metabolism
EDAT- 2001/07/07 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/07/07 10:00
PHST- 2001/07/07 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/07/07 10:00 [entrez]
AID - 10.1089/107999001750277934 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2001 Jun;21(6):445-50. doi: 
      10.1089/107999001750277934.