PMID- 11441050
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20220216
IS  - 0022-0949 (Print)
IS  - 0022-0949 (Linking)
VI  - 204
IP  - Pt 12
DP  - 2001 Jun
TI  - Expression and function of brain-derived neurotrophin factor and its receptor, 
      TrkB, in ovarian follicles from the domestic hen (Gallus gallus domesticus).
PG  - 2087-95
AB  - This report summarizes patterns of mRNA expression for the brain-derived 
      neurotrophic factor (BDNF) together with its high-affinity neurotrophin receptor 
      trkB within the hen ovary during follicle development, describes hormonal 
      mechanisms for the regulation of trkB gene expression and provides preliminary 
      evidence for a novel function for BDNF-mediated TrkB signaling within the 
      granulosa layer. Levels of BDNF mRNA in the thecal layer and of trkB mRNA within 
      the granulosa cell layer increase coincident with entrance of the follicle into 
      the preovulatory hierarchy. Localization of the BDNF mRNA transcript correlates 
      with expression of BDNF protein within the theca interna of preovulatory 
      follicles, while localization of trkB mRNA and protein occurs extensively within 
      the granulosa cell layer of preovulatory follicles. This pattern of expression 
      suggests a paracrine relationship between theca and granulosa cells for BDNF 
      signaling via TrkB. Vasoactive intestinal peptide and gonadotropin treatments 
      stimulate increases in levels of trkB mRNA within cultured granulosa cells 
      derived from both prehierarchal and preovulatory follicles, and this response is 
      increased by co-treatment with 3-isobutyl-1-methylxanthine. Finally, BDNF 
      treatment of cultured granulosa cells from preovulatory follicles results in a 
      modest, but significant, reduction in basal progesterone production, whereas this 
      effect was reversed by k252a, an inhibitor of Trk kinase activity. These results 
      support the proposals that BDNF functions as a paracrine signal in hen granulosa 
      cells and that its physiological functions may include the modulation of 
      steroidogenesis.
FAU - Jensen, T
AU  - Jensen T
AD  - Department of Biological Sciences, PO Box 369, The University of Notre Dame, 
      Notre Dame, IN 46556, USA.
FAU - Johnson, A L
AU  - Johnson AL
LA  - eng
GR  - HD36095/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Exp Biol
JT  - The Journal of experimental biology
JID - 0243705
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/pharmacology/*physiology
MH  - Chickens
MH  - Female
MH  - Gene Expression
MH  - Granulosa Cells/physiology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Ovarian Follicle/drug effects/*physiology
MH  - Progesterone/biosynthesis
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptor, trkB/*genetics/*physiology
MH  - Signal Transduction
MH  - Theca Cells/physiology
EDAT- 2001/07/07 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/07/07 10:00
PHST- 2001/07/07 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/07/07 10:00 [entrez]
AID - 10.1242/jeb.204.12.2087 [doi]
PST - ppublish
SO  - J Exp Biol. 2001 Jun;204(Pt 12):2087-95. doi: 10.1242/jeb.204.12.2087.