PMID- 11441963
OWN - NLM
STAT- MEDLINE
DCOM- 20011231
LR  - 20131121
IS  - 0145-5680 (Print)
IS  - 0145-5680 (Linking)
VI  - 47
IP  - 3
DP  - 2001 May
TI  - Lymphocyte chromosomal aberrations and their complexity induced in vitro by 
      plutonium-239 alpha-particles and detected by FISH.
PG  - 549-56
AB  - G0 human lymphocytes were exposed in vitro to plutonium-239 alpha-particles, with 
      doses ranging from 0 to 1.62 Gy, to provide a dose response curve and to compare 
      complex rearrangements produced by high LET radiation with low LET data from 
      previous work. Metaphase chromosomes 1 and 2 were painted using fluorescence in 
      situ hybridization (FISH) whole chromosome probes. All unstable and stable 
      aberrations involving the painted chromosomes were scored. The whole genome 
      corrected alpha-coefficient for dicentrics was 0.244 +/- 0.023 and for total 
      translocations 0.346 +/- 0.032, when considering simple and complex exchanges. 
      The ratio of bicoloured total translocations to bicoloured dicentrics was 1.21 
      +/- 0.15 and the ratio of 2-way to 1-way translocations was 1.73 +/- 0.27 for 
      apparently simple exchanges only. A correlation was noted between the 
      distributions of dicentrics and translocations and this applied even when the 
      complex rearrangements were removed. 20% of the observed rearrangements were 
      complex and this observation was independent of dose. Qualitatively, following 
      irradiation with alpha-particles the complex rearrangements observed were of a 
      greater complexity than seen after X- or gamma-rays. Using the Savage and Simpson 
      system to classify the complex rearrangements, the higher order complexes were 
      found to be the most common type observed. However the insertion type increased 
      while the 2F + 2G types decreased when complex rearrangements induced by 
      alpha-particles were compared to those formed after X- or gamma-irradiation.
FAU - Moquet, J E
AU  - Moquet JE
AD  - National Radiological Protection Board, Didcot, Oxon, UK. 
      jayne.moquet@nrpb.org.uk
FAU - Fernandez, J L
AU  - Fernandez JL
FAU - Edwards, A A
AU  - Edwards AA
FAU - Lloyd, D C
AU  - Lloyd DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Radioisotopes)
RN  - 53023GN24M (Plutonium)
SB  - IM
MH  - Alpha Particles/*adverse effects
MH  - Chromosome Aberrations/*radiation effects
MH  - Chromosomes, Human, Pair 1/radiation effects
MH  - Chromosomes, Human, Pair 2/radiation effects
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*cytology/*radiation effects
MH  - Male
MH  - Plutonium/*adverse effects/blood
MH  - Radiation, Ionizing
MH  - Radioisotopes/adverse effects/blood
MH  - Translocation, Genetic/radiation effects
EDAT- 2001/07/10 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/07/10 10:00
PHST- 2001/07/10 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/07/10 10:00 [entrez]
PST - ppublish
SO  - Cell Mol Biol (Noisy-le-grand). 2001 May;47(3):549-56.