PMID- 11442485
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 113
IP  - 4
DP  - 2001 Jun
TI  - Identification of both myeloid CD11c+ and lymphoid CD11c- dendritic cell subsets 
      in cord blood.
PG  - 925-31
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells described to 
      date. In human peripheral blood, both myeloid and lymphoid subsets of DCs have 
      been identified. In contrast, cord blood (CB) DCs have recently been described as 
      being exclusively of the immature CD11c- lymphoid DC subset. Using an alternative 
      method of enrichment, based on a negative selection system, both lymphoid 
      (HLA-DR+ CD123+++ CD11c- CD33-) and myeloid (HLA-DR++ CD123+ CD11c+ CD33+) DCs 
      were identified in CB. Although the majority of CB DCs showed a lymphoid 
      phenotype, a significant number of CD11c+ myeloid DCs (25.6% +/- 14.5%, n = 13) 
      were also present. Other markers, such as CD80 and CD83, were negative in both 
      subsets. Analyses of the allostimulatory capacity of both subsets showed that 
      freshly isolated CB lymphoid DCs failed to induce a potent allostimulation of 
      naive CB T cells. These features are therefore consistent with previous work 
      reporting an immature phenotype for lymphoid DCs in adult blood. The significance 
      of the inverted CD11c+/CD11c- ratio observed in CB DCs (1:3) with respect to 
      adult blood DCs (3:1) remains to be explained.
FAU - Borras, F E
AU  - Borras FE
AD  - Department of Histocompatibility and Immunogenetics, NBS - North London Centre, 
      London, UK.
FAU - Matthews, N C
AU  - Matthews NC
FAU - Lowdell, M W
AU  - Lowdell MW
FAU - Navarrete, C V
AU  - Navarrete CV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Biomarkers)
RN  - 0 (CD4 Antigens)
RN  - 0 (CD40 Antigens)
RN  - 0 (CD86 protein, human)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Integrin alphaXbeta2)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Apoptosis
MH  - B7-2 Antigen
MH  - Biomarkers/analysis
MH  - CD4 Antigens/analysis
MH  - CD40 Antigens/analysis
MH  - Dendritic Cells/*immunology
MH  - Fetal Blood/*immunology
MH  - HLA-DQ Antigens/analysis
MH  - Humans
MH  - Integrin alphaXbeta2/*analysis
MH  - Lymphocyte Culture Test, Mixed
MH  - Membrane Glycoproteins/analysis
MH  - T-Lymphocytes/immunology
EDAT- 2001/07/10 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/07/10 10:00
PHST- 2001/07/10 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/07/10 10:00 [entrez]
AID - bjh2840 [pii]
AID - 10.1046/j.1365-2141.2001.02840.x [doi]
PST - ppublish
SO  - Br J Haematol. 2001 Jun;113(4):925-31. doi: 10.1046/j.1365-2141.2001.02840.x.