PMID- 11443550
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20081121
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 184
IP  - 3
DP  - 2001 Aug 1
TI  - Human immunodeficiency virus-infected patients receiving highly active 
      antiretroviral therapy maintain activated CD8+ T cell subsets as a strong 
      adaptive immune response to cytomegalovirus.
PG  - 256-67
AB  - CD8(+) T lymphocyte function specific for human cytomegalovirus (CMV) was 
      evaluated in 14 patients infected with human immunodeficiency virus (HIV) 
      receiving highly active antiretroviral therapy (HAART) and 26 CMV-seropositive 
      donors without HIV infection. Fifty-seven percent of the HIV-infected group had 
      CMV-specific cytolytic activity in freshly isolated peripheral blood mononuclear 
      cells (PBMC) against targets expressing CMV pp65. Both interferon (IFN)-gamma 
      secretion by CD8(+) T cells and the frequency of human leukocyte antigen 
      (HLA)-tetramer-positive T cells in HLA-A*0201-positive HIV-infected subjects 
      correlated with CMV-specific cytolysis. In contrast, PBMC from healthy 
      CMV-seropositive donors did not have either measurable CMV-specific cytolysis or 
      secretion of IFN-gamma without in vitro stimulation. The T helper response to CMV 
      antigens was vigorous in healthy CMV-seropositive donors but low in the cohort of 
      HIV-infected patients. Potent CD8(+) cytotoxic T lymphocyte responses to CMV in 
      HIV-infected patients receiving HAART is the converse of what is found in healthy 
      CMV-seropositive subjects and may be the predominant adaptive immune response 
      against CMV in HIV-infected patients.
FAU - Villacres, M C
AU  - Villacres MC
AD  - Laboratory of Vaccine Research, Department of Virology, Beckman Research 
      Institute, City of Hope National Medical Center, Duarte, California, USA.
FAU - Lacey, S F
AU  - Lacey SF
FAU - La Rosa, C
AU  - La Rosa C
FAU - Krishnan, R
AU  - Krishnan R
FAU - Auge, C
AU  - Auge C
FAU - Longmate, J
AU  - Longmate J
FAU - Zaia, J A
AU  - Zaia JA
FAU - Leedom, J M
AU  - Leedom JM
FAU - Diamond, D J
AU  - Diamond DJ
LA  - eng
GR  - MO1-RR00043-38/RR/NCRR NIH HHS/United States
GR  - R01-AI43267/AI/NIAID NIH HHS/United States
GR  - R01-CA77544/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20010710
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (cytomegalovirus matrix protein 65kDa)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - AIDS-Related Opportunistic Infections/*immunology
MH  - Adult
MH  - *Antiretroviral Therapy, Highly Active
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Cross-Sectional Studies
MH  - Cytomegalovirus/*immunology
MH  - Cytomegalovirus Infections/*immunology
MH  - HIV/isolation & purification
MH  - HIV Infections/*drug therapy/*immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - *Lymphocyte Activation
MH  - Middle Aged
MH  - Phosphoproteins/immunology
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/blood
MH  - T-Lymphocyte Subsets/immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Viral Load
MH  - Viral Matrix Proteins/immunology
EDAT- 2001/07/10 10:00
MHDA- 2001/08/24 10:01
CRDT- 2001/07/10 10:00
PHST- 2000/11/14 00:00 [received]
PHST- 2001/03/28 00:00 [revised]
PHST- 2001/07/10 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/07/10 10:00 [entrez]
AID - JID001386 [pii]
AID - 10.1086/322028 [doi]
PST - ppublish
SO  - J Infect Dis. 2001 Aug 1;184(3):256-67. doi: 10.1086/322028. Epub 2001 Jul 10.