PMID- 11446853
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190906
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 88
IP  - 1
DP  - 2001 Jul
TI  - Histopathological examination of both ipsilateral and contralateral testes with 
      different obstructive models in prepubertal and adult rats.
PG  - 84-9
AB  - OBJECTIVE: To determine the histopathological changes in both the ipsilateral and 
      contralateral testes of prepubertal and adult male rats 60 days after creating 
      different obstructive models. MATERIAL AND METHODS: Thirty-six prepubertal and 32 
      adult albino male rats were examined in three different obstructive models of the 
      right testis. In group 1 the spermatic cord was ligated, in group 2 the ligation 
      was between the caput epididymis and testis, and in group 3 the vas deferens was 
      ligated. Sixty days after ligation both testes were removed and evaluated for 
      testis diameter, mean seminiferous tubule diameter (MSTD), and degenerative, 
      obstructive and inflammatory changes. RESULTS: The diameter of the obstructed 
      right testis and MSTD were significantly greater in prepubertal rats but there 
      was no apparent difference in adult rats. For obstructive changes, sloughing of 
      germ cells in the prepubertal rats and germ cell absence in adult rats were 
      significantly more common in group 3. The contralateral testis diameter and MSTD 
      of group 3 was significantly greater only in prepubertal rats. Statistically 
      significant values of obstructive change, e.g. sloughing of germ cells and apical 
      vacuolation in Sertoli cells, were apparent in prepubertal rats, but tubular 
      ectasis was the only statistically significant criterion of obstruction in adult 
      rats. CONCLUSION: The testes are more susceptible to obstruction of the vas 
      deferens in prepubertal than in adults rats, resulting in increased hydrostatic 
      pressure and oedema of both the ipsilateral and contralateral testes, which might 
      be caused by collateral circulation and rat testicular microcirculation, with a 
      rhythmic pattern of arteriolar dilatation and constriction (vasomotion). Although 
      the presence of oedema and high hydrostatic pressure was not prominent in adults, 
      formation of spermatic granulomas and absence or sloughing of germ cells in the 
      obstructed and contralateral testes reflect the early effects of vas ligation on 
      spermatogenesis in adulthood.
FAU - Altay, B
AU  - Altay B
AD  - Department of Urology, Medical School of Ege University, Izmir, Turkey. 
      baltay@mail.koc.net
FAU - Hekimgil, M
AU  - Hekimgil M
FAU - KEFi, A
AU  - KEFi A
FAU - Cikili, N
AU  - Cikili N
LA  - eng
PT  - Journal Article
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
SB  - IM
MH  - Animals
MH  - Calcinosis/pathology
MH  - Constriction, Pathologic
MH  - Granuloma/pathology
MH  - Ligation
MH  - Male
MH  - Necrosis
MH  - Rats
MH  - Spermatic Cord
MH  - Testicular Diseases/*pathology
EDAT- 2001/07/12 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/07/12 10:00
PHST- 2001/07/12 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/07/12 10:00 [entrez]
AID - bju2256 [pii]
AID - 10.1046/j.1464-410x.2001.02256.x [doi]
PST - ppublish
SO  - BJU Int. 2001 Jul;88(1):84-9. doi: 10.1046/j.1464-410x.2001.02256.x.