PMID- 11447241 OWN - NLM STAT- MEDLINE DCOM- 20010830 LR - 20190513 IS - 0022-1503 (Print) IS - 0022-1503 (Linking) VI - 92 IP - 3 DP - 2001 May-Jun TI - Production of near-isogenic lines and marked monosomic lines in common wheat (Triticum aestivum) cv. Chinese Spring. PG - 254-9 AB - Sixteen near-isogenic lines (NILs) carrying a marker gene were produced by the recurrent backcrossing method in the genetic background of common wheat (Triticum aestivum) cv. Chinese Spring (CS). Three genes from alien species showed segregation distortion. In NILs carrying a marker gene of rye (Secale cereale) or Aegilops caudata, the alien chromosome segments were detected by fluorescence in situ hybridization (FISH). The NILs were grown with replications and the effect of marker genes on plant morphology in the genetic background of CS was investigated. These NILs were further crossed with the corresponding monosomics of CS and 13 monosomic lines whose monosome carries a respective marker gene were established and named "marked monosomics." Many of the marked monosomics were distinguishable from the disomic NILs because of the different dosage effect of the genes. The NILs are utilized for studies on gene isolation or gene regulation. Marked monosomics are useful not only for monosomic analysis but also for production of homologous chromosome substitution lines because chromosome observation is not required. FAU - Tsujimoto, H AU - Tsujimoto H AD - Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama 244-0813, Japan. LA - eng PT - Journal Article PL - United States TA - J Hered JT - The Journal of heredity JID - 0375373 RN - 0 (Genetic Markers) RN - 0 (Plant Proteins) SB - IM MH - Alleles MH - Chromosome Mapping MH - Chromosome Segregation MH - *Crosses, Genetic MH - *Genetic Markers MH - Homozygote MH - In Situ Hybridization, Fluorescence MH - Monosomy MH - Morphogenesis MH - Plant Proteins/*genetics/metabolism MH - Polyploidy MH - Triticum/*genetics EDAT- 2001/07/12 10:00 MHDA- 2001/08/31 10:01 CRDT- 2001/07/12 10:00 PHST- 2001/07/12 10:00 [pubmed] PHST- 2001/08/31 10:01 [medline] PHST- 2001/07/12 10:00 [entrez] AID - 10.1093/jhered/92.3.254 [doi] PST - ppublish SO - J Hered. 2001 May-Jun;92(3):254-9. doi: 10.1093/jhered/92.3.254.