PMID- 11447248 OWN - NLM STAT- MEDLINE DCOM- 20010830 LR - 20190513 IS - 0022-1503 (Print) IS - 0022-1503 (Linking) VI - 92 IP - 3 DP - 2001 May-Jun TI - Chromosomal mapping of 18S-28S rRNA genes and 10 cDNA clones of human chromosome 1 in the musk shrew (Suncus murinus). PG - 282-7 AB - The direct R-banding fluorescence in situ hybridization (FISH) method was used to map 18S-28S ribosomal RNA genes and 10 human cDNA clones on the chromosomes of the musk shrew (Suncus murinus). The chromosomal locations of 18S-28S ribosomal RNA genes were examined in the five laboratory lines and wild animals captured in the Philippines and Vietnam, and the genes were found on chromosomes 5, 6, 9, and 13 with geographic variation. The comparative mapping of 10 cDNA clones of human chromosome 1 demonstrated that human chromosome 1 consisted of at least three segments homologous to Suncus chromosomes (chromosomes 7, 10, and 14). This approach with the direct R-banding FISH method is useful for constructing comparative maps between human and insectivore species and for explicating the process of chromosomal rearrangements during the evolution of mammals. FAU - Kuroiwa, A AU - Kuroiwa A AD - Laboratory of Animal Genetics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan. FAU - Matsubara, K AU - Matsubara K FAU - Nagase, T AU - Nagase T FAU - Nomura, N AU - Nomura N FAU - Seong, J K AU - Seong JK FAU - Ishikawa, A AU - Ishikawa A FAU - Anunciado, R V AU - Anunciado RV FAU - Tanaka, K AU - Tanaka K FAU - Yamagata, T AU - Yamagata T FAU - Masangkay, J S AU - Masangkay JS FAU - Dang, V B AU - Dang VB FAU - Namikawa, T AU - Namikawa T FAU - Matsuda, Y AU - Matsuda Y LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Hered JT - The Journal of heredity JID - 0375373 RN - 0 (DNA Probes) RN - 0 (DNA, Complementary) RN - 0 (RNA, Ribosomal, 18S) RN - 0 (RNA, Ribosomal, 28S) SB - IM MH - Animals MH - Brain MH - Chromosome Banding MH - *Chromosome Mapping MH - Chromosomes, Human, Pair 1/*genetics MH - Chromosomes, Human, Pair 10/genetics MH - Chromosomes, Human, Pair 14/genetics MH - Chromosomes, Human, Pair 7/genetics MH - DNA Probes MH - DNA, Complementary/genetics MH - Heterozygote MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - RNA, Ribosomal, 18S/*genetics MH - RNA, Ribosomal, 28S/*genetics MH - Shrews/*genetics EDAT- 2001/07/12 10:00 MHDA- 2001/08/31 10:01 CRDT- 2001/07/12 10:00 PHST- 2001/07/12 10:00 [pubmed] PHST- 2001/08/31 10:01 [medline] PHST- 2001/07/12 10:00 [entrez] AID - 10.1093/jhered/92.3.282 [doi] PST - ppublish SO - J Hered. 2001 May-Jun;92(3):282-7. doi: 10.1093/jhered/92.3.282.