PMID- 11447584
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20190906
IS  - 0021-9967 (Print)
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Linking)
VI  - 436
IP  - 4
DP  - 2001 Aug 6
TI  - TrkB-like immunoreactivity is present on geniculocortical afferents in layer IV 
      of kitten primary visual cortex.
PG  - 391-8
AB  - Exogenous administration of the neurotrophins brain-derived neurotrophic factor 
      (BDNF) or neurotrophin-4/5 (NT-4/5), or blockade of their endogenous actions, 
      have been reported to affect the anatomic organization and physiological 
      responses of neurons in developing mammalian primary visual cortex. Experimental 
      alteration of levels of these neurotrophic factors can also influence the 
      morphology of the geniculocortical afferents that project from the lateral 
      geniculate nucleus (LGN) to primary visual cortex. BDNF and NT-4/5 are ligands of 
      the TrkB tyrosine kinase receptor. Although multiple populations of cortical 
      neurons express TrkB, it is not known whether geniculocortical afferents express 
      this receptor on their axon branches in visual cortex. We have anatomically 
      labeled geniculocortical afferents of postnatal day 40 kittens with the 
      anterograde neuronal tracer Phaseolus vulgaris leucoagglutinin (PHA-L) and 
      performed double-label immunofluorescence with a panel of anti-TrkB antibodies. 
      Confocal microscopy and object-based colocalization analysis were used to measure 
      levels of TrkB-like immunoreactivity (IR) on geniculocortical afferents in layer 
      IV of primary visual cortex. By using a conservative analysis involving a 
      comparison of measured colocalization with the amount of colocalization expected 
      based on random overlap of TrkB puncta and PHA-L--labeled afferents, 3 of 5 
      anti-TrkB antibodies tested showed significant colocalization with the 
      geniculocortical axons. Results for the other two antibodies were indeterminate. 
      The indices obtained for colocalization of TrkB and geniculocortical afferents 
      were also compared with the equivalent index obtained for GAD65, a protein that 
      has a similar overall expression pattern to that of TrkB but is not expressed on 
      geniculocortical axons. This analysis indicated that TrkB was present on 
      geniculocortical axons for all five TrkB antibodies tested. TrkB-like IR was also 
      observed on neuronal somata in the LGN. These results indicate that TrkB 
      receptors on geniculocortical afferents are potential mediators of the actions of 
      BDNF and NT-4/5 in developing visual cortex.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Silver, M A
AU  - Silver MA
AD  - W.M. Keck Center for Integrative Neuroscience, Department of Physiology, 
      University of California, San Francisco, California 94143-0444, USA.
FAU - Stryker, M P
AU  - Stryker MP
LA  - eng
GR  - R01 EY002874/EY/NEI NIH HHS/United States
GR  - R37 EY002874/EY/NEI NIH HHS/United States
GR  - R37 EY002874-22/EY/NEI NIH HHS/United States
GR  - EY02874/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (leukoagglutinins, plants)
RN  - 145172-44-7 (neurotrophin 5)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Axons/physiology/ultrastructure
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cats
MH  - Fluorescent Antibody Technique
MH  - Geniculate Bodies/cytology/*physiology
MH  - Image Interpretation, Computer-Assisted
MH  - Immunohistochemistry
MH  - Nerve Growth Factors/pharmacology
MH  - Neurons, Afferent/*physiology
MH  - Neuroprotective Agents/pharmacology
MH  - Phytohemagglutinins
MH  - Receptor, trkB/*metabolism
MH  - Visual Cortex/cytology/*physiology
PMC - PMC2553095
MID - NIHMS50175
EDAT- 2001/07/12 10:00
MHDA- 2001/08/31 10:01
PMCR- 2008/09/24
CRDT- 2001/07/12 10:00
PHST- 2001/07/12 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/07/12 10:00 [entrez]
PHST- 2008/09/24 00:00 [pmc-release]
AID - 10.1002/cne.1075 [doi]
PST - ppublish
SO  - J Comp Neurol. 2001 Aug 6;436(4):391-8. doi: 10.1002/cne.1075.