PMID- 11447604
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20061115
IS  - 0749-503X (Print)
IS  - 0749-503X (Linking)
VI  - 18
IP  - 10
DP  - 2001 Jul
TI  - Cloning and characterization of a LON gene homologue from the human pathogen 
      Paracoccidioides brasiliensis.
PG  - 981-8
AB  - A LON gene homologue from the human pathogen Paracoccidioides brasiliensis 
      (PbLON) has been cloned, sequenced and characterized. It encodes a putative 
      ATP-dependent proteinase Lon, which in Saccharomyces cerevisisae (PIM1) is a 
      heat-inducible protein involved in the degradation of abnormal or short-lived 
      proteins in the mitochondria. The PbLON ORF is within a 3369 bp fragment 
      interrupted by two introns located in the 3'segment. The 5' and 3' regions 
      flanking the ORF contain sequences which resemble known transcription elements. 
      Several transcription binding factor motifs have also been found, including sites 
      for heat shock/stress response and nitrogen control. The deduced protein consists 
      of 1063 residues containing a mitochondrial import signal at the N-terminus and 
      conserved ATP-binding (GPPGVGKT) and serine catalytic (KDGPSAG) sites. It shares 
      high identity with Lon homologues from S. cerevisiae (73%), Homo sapiens (62%) 
      and Escherichia coli (56%). In P. brasiliensis, an MDJ1 putative gene has also 
      been partially sequenced adjacent to PbLON, possibly sharing divergently 
      orientated promoter elements. This chromosomal organization is interesting, since 
      Mdj1p is a heat shock chaperone essential for substrate degradation by PIM1 in 
      yeast.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Barros, T F
AU  - Barros TF
AD  - Departamento de Microbiologia, Imunologia e Parasitologia da Universidade Federal 
      de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Puccia, R
AU  - Puccia R
LA  - eng
SI  - GENBANK/AF239178
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Yeast
JT  - Yeast (Chichester, England)
JID - 8607637
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (HSP40 Heat-Shock Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (MDJ1 protein, S cerevisiae)
RN  - 0 (Membrane Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 3.4.21.- (ATP-Dependent Proteases)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.53 (Lon protein, E coli)
RN  - EC 3.4.21.53 (Protease La)
SB  - IM
MH  - ATP-Dependent Proteases
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Binding Sites
MH  - Chromosome Mapping
MH  - Chromosomes, Fungal
MH  - Cloning, Molecular
MH  - *Escherichia coli Proteins
MH  - Exons
MH  - *Genes, Fungal
MH  - HSP40 Heat-Shock Proteins
MH  - Heat-Shock Proteins/*genetics
MH  - Introns
MH  - Membrane Proteins/genetics
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Paracoccidioides/*genetics/pathogenicity
MH  - *Protease La
MH  - Protein Transport
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Serine Endopeptidases/*genetics
EDAT- 2001/07/12 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/07/12 10:00
PHST- 2001/07/12 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/07/12 10:00 [entrez]
AID - 10.1002/yea.745 [doi]
PST - ppublish
SO  - Yeast. 2001 Jul;18(10):981-8. doi: 10.1002/yea.745.