PMID- 11447756
OWN - NLM
STAT- MEDLINE
DCOM- 20010726
LR  - 20191210
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 116
IP  - 1
DP  - 2001 Jul
TI  - Multiprobe FISH for enhanced detection of bladder cancer in voided urine 
      specimens and bladder washings.
PG  - 79-86
AB  - The aim of this study was to evaluate the UroVysion (Vysis, Downers Grove, IL) 
      fluorescence in situ hybridization (FISH) test for improved detection of bladder 
      cancer in urinary specimens. Three groups of specimens were examined, including 
      voided urine specimens (1) collected before resection of bladder cancer, (2) from 
      cystoscopically negative bladders of patients with previous bladder cancer, and 
      (3) from patients with benign prostatic hyperplasia (controls). FISH positivity 
      was defined as more than 2 urothelial cells with an abnormal signal copy number 
      of at least 1 of the 4 probes. FISH was positive in 1 of 27 control specimens and 
      in 33 (73%) of 45 pTa, 12 (100%) of 12 pT1, and 13 (100%) of 13 pT2-4 tumors. The 
      results were similar in a series of 68 bladder washings. In addition, FISH of 
      voided urine specimens was positive in 5 of 10 patients with negative follow-up 
      cystoscopy results. Subsequent recurrence was found in 4 of these patients but in 
      none of 5 patients with FISH-negative results. Multiprobe FISH markedly improves 
      the sensitivity and specificity of cytology for the detection of bladder cancer 
      in urine specimens.
FAU - Bubendorf, L
AU  - Bubendorf L
AD  - Institute for Pathology, University of Basel, Basel, Switzerland.
FAU - Grilli, B
AU  - Grilli B
FAU - Sauter, G
AU  - Sauter G
FAU - Mihatsch, M J
AU  - Mihatsch MJ
FAU - Gasser, T C
AU  - Gasser TC
FAU - Dalquen, P
AU  - Dalquen P
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Prospective Studies
MH  - Prostatic Hyperplasia/pathology/urine
MH  - Therapeutic Irrigation
MH  - Urinary Bladder/*pathology
MH  - Urinary Bladder Neoplasms/*pathology/*urine
MH  - Urine/*cytology
EDAT- 2001/07/13 10:00
MHDA- 2001/07/28 10:01
CRDT- 2001/07/13 10:00
PHST- 2001/07/13 10:00 [pubmed]
PHST- 2001/07/28 10:01 [medline]
PHST- 2001/07/13 10:00 [entrez]
AID - 10.1309/K5P2-4Y8B-7L5A-FAA9 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2001 Jul;116(1):79-86. doi: 10.1309/K5P2-4Y8B-7L5A-FAA9.