PMID- 11448175
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20071115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 286
IP  - 1
DP  - 2001 Jul 20
TI  - Nef is required for efficient HIV-1 replication in cocultures of dendritic cells 
      and lymphocytes.
PG  - 225-36
AB  - Dendritic cells (DCs) are thought to play a crucial role in the pathogenesis of 
      HIV-1 infection. DCs are believed to transport virus particles to lymph nodes 
      before transfer to CD4(+) lymphocytes. We have investigated the role of Nef in 
      these processes. HIV-1 replication was examined in human immature DC-lymphocyte 
      cocultures and in DCs or lymphocytes separately. Using various R5-tropic and 
      X4-tropic HIV-1 strains and their nef-deleted (Deltanef) counterparts, we show 
      that Nef is required for optimal viral replication in immature DC-T cells 
      clusters and in T lymphocytes. Nef exerts only a marginal role on viral 
      replication in immature DCs alone as well as on virion capture by DCs, long-term 
      intracellular accumulation and transmission of X4 strains to lymphocytes. We also 
      show that wild-type and Deltanef virions are similarly processed for MHC-I 
      restricted exogenous presentation by DCs. Taken together, these results help 
      explain how HIV-1 Nef may affect viral spread and immune responses in the 
      infected host.
CI  - Copyright 2001 Academic Press.
FAU - Petit, C
AU  - Petit C
AD  - Unite Retrovirus et Transfert Genetique, Laboratoire d'Immunopathologie Virale, 
      URA CNRS 1930, Institut Pasteur, 28 rue du Dr Roux, Paris Cedex 15, 75724, 
      France.
FAU - Buseyne, F
AU  - Buseyne F
FAU - Boccaccio, C
AU  - Boccaccio C
FAU - Abastado, J P
AU  - Abastado JP
FAU - Heard, J M
AU  - Heard JM
FAU - Schwartz, O
AU  - Schwartz O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Gene Products, nef)
RN  - 0 (nef Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/pathology/*virology
MH  - Gene Products, nef/*physiology
MH  - HIV Infections/pathology/*virology
MH  - HIV-1/*physiology
MH  - Humans
MH  - Lymphocytes/pathology/*virology
MH  - Virus Replication
MH  - nef Gene Products, Human Immunodeficiency Virus
EDAT- 2001/07/13 10:00
MHDA- 2001/08/24 10:01
CRDT- 2001/07/13 10:00
PHST- 2001/07/13 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/07/13 10:00 [entrez]
AID - S0042-6822(01)90984-5 [pii]
AID - 10.1006/viro.2001.0984 [doi]
PST - ppublish
SO  - Virology. 2001 Jul 20;286(1):225-36. doi: 10.1006/viro.2001.0984.