PMID- 11448356
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20121115
IS  - 0886-4470 (Print)
IS  - 0886-4470 (Linking)
VI  - 127
IP  - 7
DP  - 2001 Jul
TI  - Expression of matrix metalloproteinases and their inhibitors correlates with 
      invasion and metastasis in squamous cell carcinoma of the head and neck.
PG  - 813-20
AB  - BACKGROUND: Matrix metalloproteinases (MMPs) have been implicated in the invasion 
      and metastasis of head and neck squamous cell carcinoma (HNSCC). However, a 
      detailed analysis of MMPs and tissue inhibitors of MMPs (TIMPs) in relation to 
      the biological behavior of HNSCC has yet to be performed in clinical material. 
      OBJECTIVES: To study a comprehensive profile of MMPs and their 2 main inhibitors 
      in HNSCC tissue samples and to correlate the patterns of expression with 
      clinicopathological characteristics, invasion, and metastasis. DESIGN: This study 
      included 54 consecutive patients with primary HNSCC, 27 of which showed lymph 
      node metastasis. Expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-10, MMP-11, 
      MMP-13, MMP-14, TIMP-1, and TIMP-2 was simultaneously analyzed in tissue 
      homogenates using semiquantitative reverse transcription-polymerase chain 
      reaction assay. Where feasible, levels of protein and enzyme activity were 
      confirmed by Western blot, enzyme-linked immunosorbent assay, and substrate 
      zymography. Conventional clinicopathological features, including mode of tumor 
      invasion, were also examined. RESULTS: Significantly higher MMP-1, MMP-2, MMP-3, 
      MMP-7, MMP-9, MMP-10, MMP-11, MMP-13, and TIMP-1 levels were found in tumors vs 
      specimens of matched normal mucosa. No difference in the distribution of MMPs and 
      TIMPs in relation to age, sex, tumor site, or histological grade was observed. A 
      significant correlation was demonstrated between levels of MMP-1, MMP-9, and 
      TIMP-1 and advanced T stage and between MMP-9 expression and an infiltrative 
      pattern of growth. Enhanced expression of MMP-9 was strongly correlated (P<.001) 
      and levels of MMP-2, MMP-7, and MMP-11 were weakly correlated (P =.03-.05) with 
      lymph node involvement. CONCLUSIONS: Overexpression of multiple MMPs and TIMPs is 
      characteristic of HNSCC, and analysis of specific MMPs, MMP-9 in particular, 
      might be useful for evaluating the malignant potential in individual HNSCC.
FAU - O-Charoenrat, P
AU  - O-Charoenrat P
AD  - Division of Head and Neck Surgery, Department of Surgery, Siriraj Hospital 
      Medical School, Bangkok 10700, Thailand. pornchaio@hotmail.com
FAU - Rhys-Evans, P H
AU  - Rhys-Evans PH
FAU - Eccles, S A
AU  - Eccles SA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Otolaryngol Head Neck Surg
JT  - Archives of otolaryngology--head & neck surgery
JID - 8603209
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Male
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases/*analysis
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - Otorhinolaryngologic Neoplasms/*pathology
MH  - Prognosis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tremor
EDAT- 2001/08/04 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/08/04 10:00
PHST- 2001/08/04 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/08/04 10:00 [entrez]
AID - ooa00225 [pii]
PST - ppublish
SO  - Arch Otolaryngol Head Neck Surg. 2001 Jul;127(7):813-20.