PMID- 11448988
OWN - NLM
STAT- MEDLINE
DCOM- 20010816
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 154
IP  - 1
DP  - 2001 Jul 9
TI  - Large-scale chromatin decondensation and recondensation regulated by 
      transcription from a natural promoter.
PG  - 33-48
AB  - We have examined the relationship between transcription and chromatin structure 
      using a tandem array of the mouse mammary tumor virus (MMTV) promoter driving a 
      ras reporter. The array was visualized as a distinctive fluorescent structure in 
      live cells stably transformed with a green fluorescent protein (GFP)-tagged 
      glucocorticoid receptor (GR), which localizes to the repeated MMTV elements after 
      steroid hormone treatment. Also found at the array by immunofluorescence were two 
      different steroid receptor coactivators (SRC1 and CBP) with acetyltransferase 
      activity, a chromatin remodeler (BRG1), and two transcription factors (NFI and 
      AP-2). Within 3 h after hormone addition, arrays visualized by GFP-GR or DNA 
      fluorescent in situ hybridization (FISH) decondensed to varying degrees, in the 
      most pronounced cases from a approximately 0.5-microm spot to form a fiber 1-10 
      microm long. Arrays later recondensed by 3-8 h of hormone treatment. The degree 
      of decondensation was proportional to the amount of transcript produced by the 
      array as detected by RNA FISH. Decondensation was blocked by two different drugs 
      that inhibit polymerase II, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole 
      (DRB) and alpha-amanitin. These observations demonstrate a role for polymerase in 
      producing and maintaining decondensed chromatin. They also support fiber-packing 
      models of higher order structure and suggest that transcription from a natural 
      promoter may occur at much higher DNA-packing densities than reported previously.
FAU - Muller, W G
AU  - Muller WG
AD  - Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, 
      National Institutes of Health, 41 Library Dr., Bethesda, MD 20892, USA.
FAU - Walker, D
AU  - Walker D
FAU - Hager, G L
AU  - Hager GL
FAU - McNally, J G
AU  - McNally JG
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Amanitins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Luminescent Proteins)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (NFI-A2 protein, rat)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factor AP-2)
RN  - 0 (Transcription Factors)
RN  - 0 (citrate-binding transport protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 53-85-0 (Dichlororibofuranosylbenzimidazole)
RN  - 9007-49-2 (DNA)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (Ncoa1 protein, mouse)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
RN  - EC 3.6.1.- (Smarca4 protein, mouse)
RN  - EC 3.6.4.- (DNA Helicases)
SB  - IM
MH  - Amanitins/pharmacology
MH  - Animals
MH  - Carrier Proteins/metabolism
MH  - Chromatin/*chemistry/*metabolism/ultrastructure
MH  - DNA/metabolism
MH  - DNA Helicases
MH  - DNA-Binding Proteins/metabolism
MH  - Dichlororibofuranosylbenzimidazole/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Genes, ras/genetics
MH  - Green Fluorescent Proteins
MH  - Histone Acetyltransferases
MH  - In Situ Hybridization, Fluorescence
MH  - Luminescent Proteins/metabolism
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - NFI Transcription Factors
MH  - Nuclear Proteins/metabolism
MH  - Nuclear Receptor Coactivator 1
MH  - *Promoter Regions, Genetic
MH  - Receptors, Glucocorticoid/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Time Factors
MH  - Transcription Factor AP-2
MH  - Transcription Factors/metabolism
MH  - *Transcription, Genetic
MH  - Transfection
PMC - PMC2196867
EDAT- 2001/07/13 10:00
MHDA- 2001/08/17 10:01
PMCR- 2002/01/09
CRDT- 2001/07/13 10:00
PHST- 2001/07/13 10:00 [pubmed]
PHST- 2001/08/17 10:01 [medline]
PHST- 2001/07/13 10:00 [entrez]
PHST- 2002/01/09 00:00 [pmc-release]
AID - 0011069 [pii]
AID - 10.1083/jcb.200011069 [doi]
PST - ppublish
SO  - J Cell Biol. 2001 Jul 9;154(1):33-48. doi: 10.1083/jcb.200011069.