PMID- 11449303
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20190605
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 34
IP  - 7
DP  - 2001 Jul
TI  - Lack of mutations of exon 2 of the MEN1 gene in endocrine and nonendocrine 
      sporadic tumors.
PG  - 861-5
AB  - In addition to the mutations that underlie most cases of the multiple endocrine 
      neoplasia type 1 (MEN1) syndrome, somatic mutations of the MEN1 gene have also 
      been described in sporadic tumors like gastrinomas, insulinomas and bronchial 
      carcinoid neoplasm. We examined exon 2 of this gene, where most of the mutations 
      have been described, in 148 endocrine and nonendocrine sporadic tumors. DNA was 
      obtained by phenol/chloroform extraction and ethanol precipitation from 92 
      formalin-fixed, paraffin-embedded samples, and from 40 fresh tumor tissue 
      samples. We used 5 pairs of primers to encompass the complete coding sequence of 
      exon 2 of the MEN1 gene that was screened by the polymerase chain 
      reaction-single-stranded conformation polymorphism (PCR-SSCP) technique in 78 
      sporadic thyroid cancers: 28 follicular adenomas, 35 papillary carcinomas, 14 
      follicular carcinomas, and 1 anaplastic thyroid carcinoma. We also examined 46 
      adrenal lesions (3 hyperplasias, 3 adenomas and 35 adrenocortical carcinomas, 2 
      pheochromocytomas, 2 ganglioneuroblastomas, and 1 lymphoma) and 24 breast cancers 
      (6 noninvasive, 16 infiltrating ductal, and 2 invasive lobular tumors). The PCR 
      product of 5 tumors suspected to present band shifts by SSCP was cloned. Direct 
      sense and antisense sequencing did not identify mutations. These results suggest 
      that the MEN1 gene is not important in breast, thyroid or adrenal sporadic 
      tumorigenesis. Because the frequency of mutations varies significantly among 
      tumor subgroups and allelic deletions are frequently observed at 11q13 in thyroid 
      and adrenal cancers, another tumor suppressor gene residing in this region is 
      likely to be involved in the tumorigenesis of these neoplasms.
FAU - Costa, S C
AU  - Costa SC
AD  - Departamento de Clinica Medica, Faculdade de Ciencias Medicas, Universidade 
      Estadual de Campinas, Campinas, SP, Brasil.
FAU - Nascimento, L S
AU  - Nascimento LS
FAU - Ferreira, F J
AU  - Ferreira FJ
FAU - Mattos, P S
AU  - Mattos PS
FAU - Camara-Lopes, L H
AU  - Camara-Lopes LH
FAU - Ward, L S
AU  - Ward LS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (DNA, Neoplasm)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
SB  - IM
MH  - Adolescent
MH  - Adrenal Gland Neoplasms/*genetics
MH  - Adult
MH  - Aged
MH  - Breast Neoplasms/*genetics
MH  - Child
MH  - Child, Preschool
MH  - DNA, Neoplasm/*analysis/genetics
MH  - Exons/*genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Multiple Endocrine Neoplasia Type 1/genetics
MH  - Mutation/*genetics
MH  - Neoplasm Proteins/*genetics
MH  - Neoplasms/*genetics
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - *Proto-Oncogene Proteins
EDAT- 2001/07/13 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/07/13 10:00
PHST- 2001/07/13 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/07/13 10:00 [entrez]
AID - S0100-879X2001000700004 [pii]
AID - 10.1590/s0100-879x2001000700004 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2001 Jul;34(7):861-5. doi: 10.1590/s0100-879x2001000700004.