PMID- 11449404
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20191105
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 46
IP  - 3
DP  - 2001 Jun 15
TI  - Distinctive patterns of Her-2/neu, c-myc, and cyclin D1 gene amplification by 
      fluorescence in situ hybridization in primary human breast cancers.
PG  - 136-49
AB  - BACKGROUND: Human solid tumors undergo clonal evolution as they progress, but 
      evidence for specific sequences of genetic changes that occur in individual 
      tumors and are recapitulated in other tumors is difficult to obtain. METHODS: 
      Patterns of amplification of Her-2/neu, c-myc, and cyclin D1 were determined by 
      fluorescence in situ hybridization (FISH) in relation to the presence of p53 
      dysfunction and ploidy in 60 primary human breast cancers. RESULTS: We show that 
      there are clusters of genophenotypic abnormalities that distinguish lobular 
      breast cancers from nonlobular tumors; that cyclin D1 amplification occurs prior 
      to the divergence of lobular breast cancers from nonlobular cancers; that p53 
      dysfunction, Her-2/neu amplification, and c-myc amplification are characteristic 
      features of nonlobular breast cancers, but not of lobular breast cancers; and 
      that the frequencies of amplification of all three oncogenes examined increase 
      progressively with increasing aneuploidy, but that each gene exhibits a different 
      profile of increasing amplification in relation to tumor progression. Early 
      amplification of c-myc appears to be an especially prominent feature of 
      hypertetraploid/hypertetrasomic tumors. CONCLUSIONS: The data suggest that in 
      tumors containing multiple abnormalities, these abnormalities often accumulate in 
      the same cells within each tumor. Furthermore, the same patterns of accumulation 
      of multiple genophenotypic abnormalities are recapitulated in different tumors.
FAU - Janocko, L E
AU  - Janocko LE
AD  - Department of Human Genetics MCP/Hahnemann University, Allegheny General 
      Hospital, Pittsburgh, Pennsylvania 15212, USA.
FAU - Brown, K A
AU  - Brown KA
FAU - Smith, C A
AU  - Smith CA
FAU - Gu, L P
AU  - Gu LP
FAU - Pollice, A A
AU  - Pollice AA
FAU - Singh, S G
AU  - Singh SG
FAU - Julian, T
AU  - Julian T
FAU - Wolmark, N
AU  - Wolmark N
FAU - Sweeney, L
AU  - Sweeney L
FAU - Silverman, J F
AU  - Silverman JF
FAU - Shackney, S E
AU  - Shackney SE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Alleles
MH  - Aneuploidy
MH  - Breast Neoplasms/*metabolism/*pathology
MH  - Carcinoma, Ductal, Breast
MH  - Chromosomes, Human, Pair 17
MH  - Cyclin D1/*biosynthesis
MH  - Disease Progression
MH  - Genes, p53/genetics
MH  - Genotype
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity
MH  - Phenotype
MH  - Ploidies
MH  - Proto-Oncogene Proteins c-myc/*biosynthesis
MH  - Receptor, ErbB-2/*biosynthesis
EDAT- 2001/07/13 10:00
MHDA- 2001/09/08 10:01
CRDT- 2001/07/13 10:00
PHST- 2001/07/13 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/07/13 10:00 [entrez]
AID - 10.1002/cyto.1098 [pii]
AID - 10.1002/cyto.1098 [doi]
PST - ppublish
SO  - Cytometry. 2001 Jun 15;46(3):136-49. doi: 10.1002/cyto.1098.