PMID- 11449988
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190915
IS  - 0065-1419 (Print)
IS  - 0065-1419 (Linking)
VI  - 76
DP  - 2000
TI  - Adrenergic mediation of TNF alpha-stimulated ICAM-1 expression on human brain 
      microvascular endothelial cells.
PG  - 117-20
AB  - Adrenergic innervation derived from locus ceruleus has been implicated in 
      regulating BBB permeability and inflammatory responses associated with 
      neurological disorders. This report demonstrates that adrenergic agents attenuate 
      the tumor necrosis factor-alpha (TNF alpha)-induced expression of intercellular 
      adhesion molecule-1 (ICAM-1) on cerebral microvascular endothelial cells (HBMEC) 
      derived from human brains. HBMEC were incubated with isoproterenol (1-10 microM) 
      alone or in the presence of propranolol (10 microM) for 30 min followed by the 
      addition of various concentrations of TNF alpha. ICAM-1 expression on cultured 
      HBMEC was dose-dependently upregulated by TNF alpha. Incubation with 
      isoproterenol significantly reduced levels of ICAM-1 expression indicating the 
      possible involvement of adrenergic agents on ICAM-1 expression. Treatment with 
      propranolol (beta 1/beta 2-adrenergic antagonist) and butoxamine (beta 
      2-adrenergic antagonist), but not atenolol (beta 1-adrenergic antagonist) 
      reversed this inhibitory effect. Isoproterenol also dose-dependently stimulated 
      cAMP production (assayed by RIA) by HBMEC; propranolol treatment abolished this 
      effect. These data show that the beta 2-adrenergic receptor/cAMP pathway may be 
      partly involved in TNF alpha-stimulated ICAM-1 expression and indicate the 
      possible involvement of adrenergic mediation of capillary function including BBB 
      integrity.
FAU - Ohara, Y
AU  - Ohara Y
AD  - Resuscitative Medicine Department, Naval Medical Research Center, Silver Spring, 
      MD, USA.
FAU - McCarron, R M
AU  - McCarron RM
FAU - Hoffman, T T
AU  - Hoffman TT
FAU - Sugano, H
AU  - Sugano H
FAU - Bembry, J
AU  - Bembry J
FAU - Lenz, F A
AU  - Lenz FA
FAU - Spatz, M
AU  - Spatz M
LA  - eng
PT  - Journal Article
PL  - Austria
TA  - Acta Neurochir Suppl
JT  - Acta neurochirurgica. Supplement
JID - 100962752
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Adrenergic Fibers/*physiology
MH  - Blood-Brain Barrier/*physiology
MH  - Brain/*blood supply
MH  - Capillary Permeability/physiology
MH  - Culture Techniques
MH  - Endothelium, Vascular/*physiopathology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/*metabolism
MH  - Locus Coeruleus/*physiopathology
MH  - Microcirculation/physiopathology
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 2001/07/14 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/07/14 10:00
PHST- 2001/07/14 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/07/14 10:00 [entrez]
AID - 10.1007/978-3-7091-6346-7_24 [doi]
PST - ppublish
SO  - Acta Neurochir Suppl. 2000;76:117-20. doi: 10.1007/978-3-7091-6346-7_24.