PMID- 11450046 OWN - NLM STAT- MEDLINE DCOM- 20010809 LR - 20190915 IS - 0065-1419 (Print) IS - 0065-1419 (Linking) VI - 76 DP - 2000 TI - Topical application of brain derived neurotrophic factor influences upregulation of constitutive isoform of heme oxygenase in the spinal cord following trauma an experimental study using immunohistochemistry in the rat. PG - 365-9 AB - Influence of brain derived neurotrophic factor (BDNF) on carbon monoxide (CO) production following spinal cord injury was examined using expression of the constitutive isoform of the enzyme hemeoxygenase-2 (HO-2) in a rat model. A longitudinal incision of the right dorsal horn on the T10-11 segment markedly increased the HO-2 immunostaining in the cord at 5 h. At this time period, breakdown of the blood-spinal cord barrier (BSCB) and edema formation were quite prominent. Repeated topical application of BDNF (20 microliters of a 1 microgram/ml solution) over the exposed surface of the cord significantly attenuated the edematous expansion of the cord and the disturbances in the BSCB permeability. In BDNF-treated rats, expression of HO-2 immunoreactivity was considerably reduced. These results strongly suggest that BDNF is neuroprotective in spinal trauma and this growth factor has the capacity to attenuate CO production by downregulating HO-2 expression. FAU - Sharma, H S AU - Sharma HS AD - Laboratory of Neuroanatomy, Department of Medical Cell Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden. FAU - Westman, J AU - Westman J FAU - Gordh, T AU - Gordh T FAU - Alm, P AU - Alm P LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Austria TA - Acta Neurochir Suppl JT - Acta neurochirurgica. Supplement JID - 100962752 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Isoenzymes) RN - 7U1EE4V452 (Carbon Monoxide) RN - EC 1.14.14.18 (Heme Oxygenase (Decyclizing)) RN - EC 1.14.14.18 (heme oxygenase-2) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*pharmacology MH - Carbon Monoxide/metabolism MH - Edema/*pathology MH - Heme Oxygenase (Decyclizing)/*metabolism MH - Isoenzymes/*metabolism MH - Male MH - Rats MH - Rats, Sprague-Dawley MH - Spinal Cord Injuries/*pathology MH - Up-Regulation/drug effects EDAT- 2001/07/14 10:00 MHDA- 2001/08/10 10:01 CRDT- 2001/07/14 10:00 PHST- 2001/07/14 10:00 [pubmed] PHST- 2001/08/10 10:01 [medline] PHST- 2001/07/14 10:00 [entrez] AID - 10.1007/978-3-7091-6346-7_76 [doi] PST - ppublish SO - Acta Neurochir Suppl. 2000;76:365-9. doi: 10.1007/978-3-7091-6346-7_76.