PMID- 11450846
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20191210
IS  - 1434-5161 (Print)
IS  - 1434-5161 (Linking)
VI  - 46
IP  - 7
DP  - 2001
TI  - Definition of a 1-Mb homozygous deletion at 9q32-q33 in a human bladder-cancer 
      cell line.
PG  - 372-7
AB  - We performed detailed molecular analyses of a suspected homozygous deletion on 
      chromosome 9q32-q33 in a bladder-cancer cell line (KYBTDS) derived from a 
      superficial papillary transitional cell carcinoma (TCC). We examined 13 
      sequence-tagged site (STS) markers mapped along 9q32-q33 by polymerase chain 
      reaction (PCR), and used 13 bacterial artificial chromosome (BAC)/bacteriophage 
      P1-derived artificial chromosome (PAC) genomic clone probes representing these 
      STS markers as probes for dual-color fluorescence in situ hybridization (FISH) 
      analyses to define the deleted region cytogenetically and at the molecular level. 
      Southern blotting confirmed the findings. This combination of techniques revealed 
      that the homozygous deletion in the KYBTDS cell line involved less than 1 
      megabase of DNA, flanked by markers A003P42 and SGC33380. This interval overlaps 
      part of a common region of deletion observed in a number of primary bladder 
      cancers; moreover, the DNA sequence within the 1-Mb segment corresponds to part 
      of a YAC genomic clone that encompasses a putative tumor suppressor gene, DBCCR1.
FAU - Fujiwara, H
AU  - Fujiwara H
AD  - Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, 
      Kawasaki, Japan.
FAU - Emi, M
AU  - Emi M
FAU - Nagai, H
AU  - Nagai H
FAU - Ohgaki, K
AU  - Ohgaki K
FAU - Imoto, I
AU  - Imoto I
FAU - Akimoto, M
AU  - Akimoto M
FAU - Ogawa, O
AU  - Ogawa O
FAU - Habuchi, T
AU  - Habuchi T
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Hum Genet
JT  - Journal of human genetics
JID - 9808008
RN  - 0 (BRINP1 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Genetic Markers)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Blotting, Southern
MH  - Cell Cycle Proteins
MH  - *Chromosome Deletion
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Expressed Sequence Tags
MH  - Genetic Markers
MH  - *Homozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity/genetics
MH  - Nerve Tissue Proteins
MH  - Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Proteins/genetics
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 2001/07/14 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/07/14 10:00
PHST- 2001/07/14 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/07/14 10:00 [entrez]
AID - 10.1007/s100380170056 [doi]
PST - ppublish
SO  - J Hum Genet. 2001;46(7):372-7. doi: 10.1007/s100380170056.