PMID- 11451178
OWN - NLM
STAT- MEDLINE
DCOM- 20020114
LR  - 20221207
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 34
IP  - 6
DP  - 2001 Jun
TI  - Identification of hepatitis B virus-specific CTL epitopes presented by 
      HLA-A*2402, the most common HLA class I allele in East Asia.
PG  - 922-9
AB  - BACKGROUND/AIMS: The aim of this study was to identify and characterize hepatitis 
      B virus (HBV)-specific cytotoxic T lymphocytes (CTL) epitopes presented by human 
      leukocyte antigen (HLA)-A*2402, most common HLA class I allele in East Asia. 
      METHODS: HLA-A*2402-restricted CTL epitopes were identified by reverse 
      immunogenetics. Immunogenecity of these epitopes was investigated using 
      peripheral blood mononuclear cell (PBMC) from HLA-A24+ patients with acute 
      hepatitis B. RESULTS: An HLA-A*2402 stabilization assay demonstrated that 36 of 
      63 HBV peptides carrying HLA-A*2402 anchor residues have high- and 
      medium-HLA-A*2402 binding affinity. Two (C117-125 and P756-764) of the 36 
      peptides induced peptide-specific CTLs. CTL clones and lines specific for these 
      peptides killed HBV recombinant vaccinia virus-infected target cells expressing 
      HLA-A*2402, indicating that these two peptides are CTL epitopes presented by 
      HLA-A*2402. These two peptides were able to induce specific CTLs in 7 and 11 of 
      12 HLA-A24+ patients with acute hepatitis B, respectively. CONCLUSIONS: We 
      identified two immunodominant CTL epitopes restricted by HLA-A*2402. Because 
      HLA-A*2402 is the most common allele in East Asia, a region in which there are 
      approximately 200 million HBV carriers, these epitopes will be useful for 
      analysis of CTL responses in patients from East Asia.
FAU - Sobao, Y
AU  - Sobao Y
AD  - Division of Viral Immunology, Center for AIDS Research, Kumamoto University, 
      Japan.
FAU - Sugi, K
AU  - Sugi K
FAU - Tomiyama, H
AU  - Tomiyama H
FAU - Saito, S
AU  - Saito S
FAU - Fujiyama, S
AU  - Fujiyama S
FAU - Morimoto, M
AU  - Morimoto M
FAU - Hasuike, S
AU  - Hasuike S
FAU - Tsubouchi, H
AU  - Tsubouchi H
FAU - Tanaka, K
AU  - Tanaka K
FAU - Takiguch, M
AU  - Takiguch M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
RN  - 0 (Hepatitis B Antigens)
RN  - 0 (Immunodominant Epitopes)
SB  - IM
MH  - Acute Disease
MH  - Alleles
MH  - Antigen Presentation
MH  - Clone Cells
MH  - Asia, Eastern
MH  - HLA-A Antigens/genetics/*metabolism
MH  - HLA-A24 Antigen
MH  - Hepatitis B/genetics/*immunology/virology
MH  - Hepatitis B Antigens/*metabolism
MH  - Hepatitis B, Chronic/genetics/immunology/virology
MH  - Humans
MH  - Immunodominant Epitopes/metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2001/07/14 10:00
MHDA- 2002/01/15 10:01
CRDT- 2001/07/14 10:00
PHST- 2001/07/14 10:00 [pubmed]
PHST- 2002/01/15 10:01 [medline]
PHST- 2001/07/14 10:00 [entrez]
AID - S0168827801000484 [pii]
AID - 10.1016/s0168-8278(01)00048-4 [doi]
PST - ppublish
SO  - J Hepatol. 2001 Jun;34(6):922-9. doi: 10.1016/s0168-8278(01)00048-4.