PMID- 11451427
OWN - NLM
STAT- MEDLINE
DCOM- 20010920
LR  - 20191105
IS  - 0166-445X (Print)
IS  - 0166-445X (Linking)
VI  - 54
IP  - 1-2
DP  - 2001 Sep
TI  - Specific expression of cytochrome P4501A1 gene in gill, intestine and liver of 
      tilapia exposed to coastal sediments.
PG  - 69-80
AB  - Toxicological effects of persistent organic pollutants (POPs) in aquatic 
      ecosystems lead to the deterioration of water quality and adversely affect fish 
      and human health. The highly lipophilic nature of these pollutants may enter fish 
      through the diet or by water-borne exposure. In monitoring contamination in 
      aquatic systems, induction of the cytochrome P450 1A1 (CYP1A1) gene of fish has 
      been evaluated as a sensitive, "early warning" method. The objective of the 
      present study was to characterize the induction of the gene in fish upon exposure 
      to coastal sediments and to determine its specific expression in liver and 
      extrahepatic organs (i.e. gill and intestine) in which the toxicological effects 
      to the corresponding tissues could be addressed. Sediment samples were collected 
      from different sites, including Victoria Harbour (VS), Ma Wan (MW), Tsim Bei Tsui 
      (TBT) and Mai Po (MP). The samples were analyzed for polychlorinated biphenyls 
      (PCBs) and polycyclic aromatic hydrocarbons (PAHs). CYP1A1 mRNA expression was 
      measured in juvenile tilapia exposed experimentally to coastal sediment for 3 and 
      7 days. A negative control group of fish maintained in seawater was used. Using 
      the primer dropping polymerase chain reaction technique, gill, intestinal and 
      hepatic CYP1A1 mRNAs were quantified. Chemical analysis shows that the samples 
      from VS contaminated with the highest concentration of PCBs (45.24 p.p.b.) and 
      PAHs (1663.7 p.p.b.), followed by MW (16.01 and 347.7 p.p.b.), TBT (14.48 and 
      235.2 p.p.b.) and MP (14.60 and 242.2 p.p.b.). Fish exposed to sediments were 
      contaminated with various levels of PCBs (VS, 64.14-72.06 p.p.b.; MP, 27.06-31.62 
      p.p.b.; TBT, 27.29-33.92 p.p.b.; MW, 16.05-17.76 p.p.b.) and PAHs (VS, 
      124.7-304.9 p.p.b.; MP, 97.57-164.1 p.p.b.; TBT, 25.38-98 p.p.b.; MW, 24.07-68.13 
      p.p.b.). The control fish displayed moderate expression of CYP1A1 mRNA in liver 
      (1.45 arbitrary units), gill (1.21 arbitrary units) and intestine (0.56 arbitrary 
      units). Following sediment exposure, there was a large increase in CYP1A1 mRNA in 
      intestine and liver but no significant changes in gill tissues. In the first 3 
      days, there was anywhere from 34-96 and 69-156% induction of the CYP1A1 
      transcripts in intestine and liver, respectively. Following 7 days of exposure, a 
      continued induction of high level of CYP1A1 mRNA in intestine (73-157%) was 
      observed. The induction of CYP1A1 in liver and intestine provided a defensive 
      mechanism against POPs entering from the external environment.
FAU - Wong, C K
AU  - Wong CK
AD  - Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong. 
      ckcwong@hkbu.edu.hk
FAU - Yeung, H Y
AU  - Yeung HY
FAU - Woo, P S
AU  - Woo PS
FAU - Wong, M H
AU  - Wong MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Cytochrome P-450 CYP1A1/*genetics
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - *Geologic Sediments
MH  - Gills/*enzymology
MH  - Intestines/*enzymology
MH  - Liver/*enzymology
MH  - Polychlorinated Biphenyls/analysis
MH  - Polycyclic Aromatic Hydrocarbons/analysis
MH  - RNA, Messenger/analysis
MH  - Tilapia/*metabolism
MH  - Water Pollutants, Chemical/*toxicity
EDAT- 2001/07/14 10:00
MHDA- 2001/09/21 10:01
CRDT- 2001/07/14 10:00
PHST- 2001/07/14 10:00 [pubmed]
PHST- 2001/09/21 10:01 [medline]
PHST- 2001/07/14 10:00 [entrez]
AID - S0166-445X(00)00173-9 [pii]
AID - 10.1016/s0166-445x(00)00173-9 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2001 Sep;54(1-2):69-80. doi: 10.1016/s0166-445x(00)00173-9.